Carbamoyl phosphate synthetase
From Proteopedia
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| - | <StructureSection load='1a9x' size='350' side='right' scene='49/493669/Cv/ | + | <StructureSection load='1a9x' size='350' side='right' scene='49/493669/Cv/4' caption='E. coli carbamoyl phosphate synthetase large chain (cyan) and small chain (salmon) complex with ADP, phosphate and ornithine, [[1a9x]]'> |
== Function == | == Function == | ||
| - | '''Carbamoyl phosphate synthetase''' (CPS) catalyzes the production of carbamoyl phosphate from ATP, Mg+2, bicarbonate | + | '''Carbamoyl phosphate synthetase''' (CPS) catalyzes the production of carbamoyl phosphate from ATP, Mg+2, bicarbonate and glutamine. CPS is part of the pyrimidine and arginine biosynthesis as well as the urea cycle in vertebrates. Ornithine is an allosteric effector of CPS.<ref>PMID:5329589</ref> There are 3 forms of CPS:<br /> |
* '''CPS I''' participates in the urea cycle. CPS I uses glutamine for NH3 source.<br /> | * '''CPS I''' participates in the urea cycle. CPS I uses glutamine for NH3 source.<br /> | ||
* '''CPS II''' participates in pyrimidine metabolism<br /> | * '''CPS II''' participates in pyrimidine metabolism<br /> | ||
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== Structural highlights == | == Structural highlights == | ||
| - | CPS I and II are composed of 2 subunits. The large subunit contain active sites which bind nucleotides and other effectors. The small subunit catalyzes the hydrolysis of glutamine to glutamate and NH3. The <scene name='49/493669/Cv/ | + | CPS I and II are composed of 2 subunits. The large subunit contain active sites which bind nucleotides and other effectors. The small subunit catalyzes the hydrolysis of glutamine to glutamate and NH3. The <scene name='49/493669/Cv/3'>small subunit active site contains an active Cys residue</scene>.<ref>PMID:9636022</ref> CPS I contains a methylglyoxal synthetase (MGS) domain which binds <scene name='49/493669/Cv/6'>L-ornithine</scene>. Water molecules are shown as red spheres. |
| - | </ | + | |
| + | <scene name='49/493669/Cv/11'>ADP binding site</scene>. | ||
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| + | <scene name='49/493669/Cv/12'>Mn coordination site</scene>. | ||
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| + | <scene name='49/493669/Cv/13'>K coordination site</scene>. | ||
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==3D structures of carbamoyl phosphate synthetase== | ==3D structures of carbamoyl phosphate synthetase== | ||
| + | [[Carbamoyl phosphate synthetase 3D structures]] | ||
| - | + | </StructureSection> | |
| - | [[2yvq]] – CPS I MGS domain - human<br /> | ||
| - | [[1jdb]] - EcCPS + phosphate + glutamine + ADP – ''Escherichia coli''<br /> | ||
| - | [[1a9x]], [[1cs0]] - EcCPS + phosphate + ornithine + ADP<BR /> | ||
| - | [[1c30]], [[1m6v]] - EcCPS (mutant) + phosphate + ornithine + ADP<BR /> | ||
| - | [[1bxr]] - EcCPS + ornithine + AMPPNP<br /> | ||
| - | [[1ce8]] - EcCPS + phosphate + ornithine + inosinic acid + ADP<BR /> | ||
| - | [[1c3o]] - EcCPS (mutant) + phosphate + ornithine + glutamine + ADP<BR /> | ||
| - | [[1kee]] - EcCPS + phosphate + ornithine + acivicin + ADP<BR /> | ||
| - | [[1t36]] - EcCPS (mutant) + phosphate + ornithine + UMP + ADP<BR /> | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Kalman SM, Duffield PH, Brzozowski T. Purification and properties of a bacterial carbamyl phosphate synthetase. J Biol Chem. 1966 Apr 25;241(8):1871-7. PMID:5329589
- ↑ Thoden JB, Miran SG, Phillips JC, Howard AJ, Raushel FM, Holden HM. Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis. Biochemistry. 1998 Jun 23;37(25):8825-31. PMID:9636022 doi:http://dx.doi.org/10.1021/bi9807761
