6h0r
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6h0r is ON HOLD Authors: Heber, S., Janowski, R., Niessing, D. Description: X-ray structure of SRS2 fragment of Rgs4 3' UTR [[Category: Unreleased ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray structure of SRS2 fragment of Rgs4 3' UTR== | |
| + | <StructureSection load='6h0r' size='340' side='right'caption='[[6h0r]], [[Resolution|resolution]] 1.73Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6h0r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H0R FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h0r OCA], [http://pdbe.org/6h0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h0r RCSB], [http://www.ebi.ac.uk/pdbsum/6h0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h0r ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Throughout metazoans, Staufen (Stau) proteins are core factors of mRNA localization particles. They consist of three to four double-stranded RNA binding domains (dsRBDs) and a C-terminal dsRBD-like domain. Mouse Staufen2 (mStau2)-like Drosophila Stau (dmStau) contains four dsRBDs. Existing data suggest that only dsRBDs 3-4 are necessary and sufficient for mRNA binding. Here, we show that dsRBDs 1 and 2 of mStau2 bind RNA with similar affinities and kinetics as dsRBDs 3 and 4. While RNA binding by these tandem domains is transient, all four dsRBDs recognize their target RNAs with high stability. Rescue experiments in Drosophila oocytes demonstrate that mStau2 partially rescues dmStau-dependent mRNA localization. In contrast, a rescue with mStau2 bearing RNA-binding mutations in dsRBD1-2 fails, confirming the physiological relevance of our findings. In summary, our data show that the dsRBDs 1-2 play essential roles in the mRNA recognition and function of Stau-family proteins of different species. | ||
| - | + | Staufen2-mediated RNA recognition and localization requires combinatorial action of multiple domains.,Heber S, Gaspar I, Tants JN, Gunther J, Moya SMF, Janowski R, Ephrussi A, Sattler M, Niessing D Nat Commun. 2019 Apr 10;10(1):1659. doi: 10.1038/s41467-019-09655-3. PMID:30971701<ref>PMID:30971701</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6h0r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Heber, S]] | [[Category: Heber, S]] | ||
| - | [[Category: Niessing, D]] | ||
[[Category: Janowski, R]] | [[Category: Janowski, R]] | ||
| + | [[Category: Niessing, D]] | ||
| + | [[Category: Rgs4]] | ||
| + | [[Category: Rna]] | ||
Current revision
X-ray structure of SRS2 fragment of Rgs4 3' UTR
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Categories: Large Structures | Heber, S | Janowski, R | Niessing, D | Rgs4 | Rna
