CCA-adding enzyme

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<StructureSection load='3ovs' size='450' side='right' caption='Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry [[3ovs]])' scene='55/551188/Cv/1'>
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<StructureSection load='3ovs' size='350' side='right' caption='Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry [[3ovs]])' scene='55/551188/Cv/1'>
'''CCA-adding enzyme''' (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA. CAE has one active site which is capable of binding CTP and ATP in a specific sequence. In the bacteria Aquifex aeolicus, CAE consists of 2 subunits. One specific for CTP and the other for ATP. CAE builds the CCA without a genetic template.<ref>PMID:15295590</ref>
'''CCA-adding enzyme''' (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA. CAE has one active site which is capable of binding CTP and ATP in a specific sequence. In the bacteria Aquifex aeolicus, CAE consists of 2 subunits. One specific for CTP and the other for ATP. CAE builds the CCA without a genetic template.<ref>PMID:15295590</ref>
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*<scene name='55/551188/Cv/2'>Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, ethanediol and Ca+2 ion</scene> (PDB entry [[3ovs]]).
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*<scene name='55/551188/Cv/4'>CCA-adding enzyme complex with tRNA, CTP, ethanediol and Ca+2 ion</scene> (PDB entry [[3ovs]]).
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*<scene name='55/551188/Cv/3'>Binding site of CCA-adding enzyme</scene> (PDB entry [[3ovs]]).<ref>PMID:21071662</ref>
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*<scene name='55/551188/Cv/5'>Ca+2 coordination site of CCA-adding enzyme</scene> (PDB entry [[3ovs]]).<ref>PMID:21071662</ref>
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</StructureSection>
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==3D structures of CCA-adding enzyme==
==3D structures of CCA-adding enzyme==
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[[CCA-adding enzyme 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*CCA-adding enzyme
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**[[1miv]] – BsCAE – ''Bacillus stearothermophilus''<BR />
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**[[1uet]], [[1r8a]] – AfCAE – ''Archaeoglobus fulgidus''<BR />
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**[[1ou5]], [[4x4w]] – hCAE – human<br />
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*CCA-adding enzyme binary complex with nucleotide
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**[[1miw]] – BsCAE + ATP<BR />
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**[[1uev]], [[1r8b]] – AfCAE + ATP<BR />
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**[[1miy]] – BsCAE + CTP<BR />
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**[[1ueu]], [[1r89]] – AfCAE + CTP<BR />
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**[[1r8c]] – AfCAE + UTP<BR />
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*CCA-adding enzyme binary complex with tRNA
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**[[2dr5]] – AfCAE + tRNA 32-mer<BR />
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**[[2dr7]], [[2zh1]], [[2zh7]], [[2zh9]], [[4x4q]], [[4x4t]], [[4x4u]] – AfCAE + tRNA 33-mer<BR />
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**[[2dr9]], [[2zh2]], [[2zh3]], [[2zh4]], [[2zh5]], [[2zh8]], [[2zhb]] – AfCAE + tRNA 34-mer<BR />
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**[[2drb]], [[3ouy]], [[4x4p]] – AfCAE + tRNA 35-mer<BR />
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*CCA-adding enzyme ternary complex with nucleotide and tRNA
 
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**[[1sz1]] – AfCAE + GTP + UMP + CMP + tRNA<BR />
 
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**[[1tfw]] – AfCAE + ATP + tRNA 3’ end<BR />
 
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**[[1tfy]] – AfCAE + CTP + tRNA 3’ end<BR />
 
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**[[2dr8]], [[2zha]], [[4x4s]] – AfCAE + CTP + tRNA 33-mer<BR />
 
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**[[4x4r]], [[4x4v]] – AfCAE + AMPCPP + tRNA 33-mer<BR />
 
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**[[4x4n]] – AfCAE + GTP + tRNA 33-mer<BR />
 
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**[[2dra]], [[2zh6]], [[3ov7]], [[4x4o]] – AfCAE + ATP + tRNA 34-mer<BR />
 
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**[[3ova]] – AfCAE + ATP derivative + tRNA 34-mer<BR />
 
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**[[2dvi]], [[3ovs]] – AfCAE + CTP + tRNA 34-mer<BR />
 
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**[[3ovb]] – AfCAE + ATP + tRNA 35-mer<BR />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry 3ovs)

Drag the structure with the mouse to rotate


References

  1. ↑ Xiong Y, Steitz TA. Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template. Nature. 2004 Aug 5;430(7000):640-5. PMID:15295590 doi:http://dx.doi.org/10.1038/nature02711
  2. ↑ Pan B, Xiong Y, Steitz TA. How the CCA-adding enzyme selects adenine over cytosine at position 76 of tRNA. Science. 2010 Nov 12;330(6006):937-40. PMID:21071662 doi:10.1126/science.1194985

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Alexander Berchansky, Michal Harel

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