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4xf2

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Revision as of 07:05, 29 May 2019

Tetragonal structure of Arp2/3 complex

Structural highlights

4xf2 is a 14 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ARPC4_BOVIN] Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament (By similarity). [ARPC2_BOVIN] Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament (By similarity). [ARP3_BOVIN] Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity). [ARC1B_BOVIN] Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity). [ARPC5_BOVIN] Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity). [ARP2_BOVIN] Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. [ARPC3_BOVIN] Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity).

Publication Abstract from PubMed

Co-crystals of the bovine Arp2/3 complex with the CA motif from N-WASP in two new space groups were analyzed by X-ray diffraction. The crystals in the orthorhombic space group P212121 contained one complex per asymmetric unit, with unit-cell parameters a = 105.48, b = 156.71, c = 177.84 A, and diffracted to 3.9 A resolution. The crystals in the tetragonal space group P41 contained two complexes per asymmetric unit, with unit-cell parameters a = b = 149.93, c = 265.91 A, and diffracted to 5.0 A resolution. The electron-density maps of both new crystal forms had densities for small segments of subdomains 1 and 2 of Arp2. Both maps had density at the binding site on Arp3 for the C-terminal EWE tripeptide from N-WASP and a binding site proposed for the C motif of N-WASP in the barbed-end groove of Arp2. The map from the tetragonal crystal form had density near the barbed end of Arp3 that may correspond to the C helix of N-WASP. The noise levels and the low resolution of the maps made the assignment of specific molecular structures for any of these CA peptides impossible.

Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites.,Jurgenson CT, Pollard TD Acta Crystallogr F Struct Biol Commun. 2015 Sep;71(Pt 9):1161-8. doi:, 10.1107/S2053230X15013515. Epub 2015 Aug 25. PMID:26323303^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jurgenson CT, Pollard TD. Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites. Acta Crystallogr F Struct Biol Commun. 2015 Sep;71(Pt 9):1161-8. doi:, 10.1107/S2053230X15013515. Epub 2015 Aug 25. PMID:26323303 doi:http://dx.doi.org/10.1107/S2053230X15013515

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xf2"

Categories: Bos taurus | Large Structures | Jurgenson, C T | Pollard, T P | Structural protein

@@ Line 1: / Line 1: @@
 ==Tetragonal structure of Arp2/3 complex==
-<StructureSection load='4xf2' size='340' side='right' caption='[[4xf2]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
+<StructureSection load='4xf2' size='340' side='right'caption='[[4xf2]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4xf2]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XF2 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xf2 OCA], [http://pdbe.org/4xf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4xf2 PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xf2 OCA], [http://pdbe.org/4xf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xf2 RCSB], [http://www.ebi.ac.uk/pdbsum/4xf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xf2 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 </StructureSection>
 [[Category: Bos taurus]]
+[[Category: Large Structures]]
 [[Category: Jurgenson, C T]]
 [[Category: Pollard, T P]]
 [[Category: Structural protein]]

4xf2

From Proteopedia

Revision as of 07:05, 29 May 2019

Tetragonal structure of Arp2/3 complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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