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4xe4

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Revision as of 07:19, 29 May 2019

Coagulation Factor XII protease domain crystal structure

Structural highlights

4xe4 is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	F12 (HUMAN)
Activity:	Coagulation factor XIIa, with EC number 3.4.21.38
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[FA12_HUMAN] Congenital factor XII deficiency;Hereditary angioedema type 3. Defects in F12 are the cause of factor XII deficiency (FA12D) [MIM:234000]; also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8] ^[9] Defects in F12 are the cause of hereditary angioedema type 3 (HAE3) [MIM:610618]; also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g. during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal.^[10] ^[11]

Function

[FA12_HUMAN] Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.^[12]

Publication Abstract from PubMed

BACKGROUND: Coagulation factor XII (FXII) is a serine protease important to kinin generation and blood coagulation cleaving substrates plasma kallikrein and factor XI (FXI). OBJECTIVE: To investigate FXII zymogen activation and substrate recognition we determined the crystal structure of the FXII protease domain. METHODS AND RESULTS: A series of recombinant FXII protease constructs were characterized measuring cleavage of chromogenic peptide and plasma kallikrein protein substrates. This revealed that the FXII protease construct spanning the light chain has unexpectedly weak proteolytic activity compared to beta-FXIIa which has an additional 9 amino acid remnant of the heavy chain present. Consistent with this data the crystal structure of the light chain protease reveals a zymogen conformation for active site residues Gly193 and Ser195 where the oxyanion hole is absent. The Asp194 side chain salt bridges to Arg73 is an atypical conformation of the 70-loop. In one crystal form the S1 pocket loops are partially flexible which is typical of a zymogen. In a second crystal form of the deglycosylated light chain the S1 pocket loops are ordered and a short alpha-helix in the 180-loop of the structure results in an enlarged and distorted S1 pocket with a buried conformation of residue Asp189 critical for P1 Arg substrate recognition. The FXII structures defines patches of negative charge surrounding the active site cleft which may be critical for interactions with inhibitors and substrates. CONCLUSIONS: This data provides the first structural scaffold for understanding FXII substrate recognition and zymogen activation. This article is protected by copyright. All rights reserved.

Coagulation Factor XII protease domain crystal structure.,Pathak M, Wilmann P, Awford J, Li C, Fischer PM, Dreveny I, Dekker LV, Emsley J J Thromb Haemost. 2015 Jan 20. doi: 10.1111/jth.12849. PMID:25604127^[13]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schloesser M, Hofferbert S, Bartz U, Lutze G, Lammle B, Engel W. The novel acceptor splice site mutation 11396(G-->A) in the factor XII gene causes a truncated transcript in cross-reacting material negative patients. Hum Mol Genet. 1995 Jul;4(7):1235-7. PMID:8528215
↑ Bernardi F, Marchetti G, Patracchini P, del Senno L, Tripodi M, Fantoni A, Bartolai S, Vannini F, Felloni L, Rossi L, et al.. Factor XII gene alteration in Hageman trait detected by TaqI restriction enzyme. Blood. 1987 May;69(5):1421-4. PMID:2882793
↑ Miyata T, Kawabata S, Iwanaga S, Takahashi I, Alving B, Saito H. Coagulation factor XII (Hageman factor) Washington D.C.: inactive factor XIIa results from Cys-571----Ser substitution. Proc Natl Acad Sci U S A. 1989 Nov;86(21):8319-22. PMID:2510163
↑ Hovinga JK, Schaller J, Stricker H, Wuillemin WA, Furlan M, Lammle B. Coagulation factor XII Locarno: the functional defect is caused by the amino acid substitution Arg 353-->Pro leading to loss of a kallikrein cleavage site. Blood. 1994 Aug 15;84(4):1173-81. PMID:8049433
↑ Schloesser M, Zeerleder S, Lutze G, Halbmayer WM, Hofferbert S, Hinney B, Koestering H, Lammle B, Pindur G, Thies K, Kohler M, Engel W. Mutations in the human factor XII gene. Blood. 1997 Nov 15;90(10):3967-77. PMID:9354665
↑ Kondo S, Tokunaga F, Kawano S, Oono Y, Kumagai S, Koide T. Factor XII Tenri, a novel cross-reacting material negative factor XII deficiency, occurs through a proteasome-mediated degradation. Blood. 1999 Jun 15;93(12):4300-8. PMID:10361128
↑ Kanaji T, Kanaji S, Osaki K, Kuroiwa M, Sakaguchi M, Mihara K, Niho Y, Okamura T. Identification and characterization of two novel mutations (Q421 K and R123P) in congenital factor XII deficiency. Thromb Haemost. 2001 Dec;86(6):1409-15. PMID:11776307
↑ Ishii K, Oguchi S, Moriki T, Yatabe Y, Takeshita E, Murata M, Ikeda Y, Watanabe K. Genetic analyses and expression studies identified a novel mutation (W486C) as a molecular basis of congenital coagulation factor XII deficiency. Blood Coagul Fibrinolysis. 2004 Jul;15(5):367-73. PMID:15205584
↑ Oguchi S, Ishii K, Moriki T, Takeshita E, Murata M, Ikeda Y, Watanabe K. Factor XII Shizuoka, a novel mutation (Ala392Thr) identified and characterized in a patient with congenital coagulation factor XII deficiency. Thromb Res. 2005;115(3):191-7. PMID:15617741 doi:10.1016/j.thromres.2004.08.027
↑ Dewald G, Bork K. Missense mutations in the coagulation factor XII (Hageman factor) gene in hereditary angioedema with normal C1 inhibitor. Biochem Biophys Res Commun. 2006 May 19;343(4):1286-9. PMID:16638441 doi:S0006-291X(06)00611-5
↑ Cichon S, Martin L, Hennies HC, Muller F, Van Driessche K, Karpushova A, Stevens W, Colombo R, Renne T, Drouet C, Bork K, Nothen MM. Increased activity of coagulation factor XII (Hageman factor) causes hereditary angioedema type III. Am J Hum Genet. 2006 Dec;79(6):1098-104. Epub 2006 Oct 18. PMID:17186468 doi:S0002-9297(07)63472-7
↑ MacQuarrie JL, Stafford AR, Yau JW, Leslie BA, Vu TT, Fredenburgh JC, Weitz JI. Histidine-rich glycoprotein binds factor XIIa with high affinity and inhibits contact-initiated coagulation. Blood. 2011 Apr 14;117(15):4134-41. doi: 10.1182/blood-2010-07-290551. Epub 2011 , Feb 8. PMID:21304106 doi:10.1182/blood-2010-07-290551
↑ Pathak M, Wilmann P, Awford J, Li C, Fischer PM, Dreveny I, Dekker LV, Emsley J. Coagulation Factor XII protease domain crystal structure. J Thromb Haemost. 2015 Jan 20. doi: 10.1111/jth.12849. PMID:25604127 doi:http://dx.doi.org/10.1111/jth.12849

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xe4"

@@ Line 1: / Line 1: @@
 ==Coagulation Factor XII protease domain crystal structure==
-<StructureSection load='4xe4' size='340' side='right' caption='[[4xe4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+<StructureSection load='4xe4' size='340' side='right'caption='[[4xe4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4xe4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XE4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4xe4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XE4 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">F12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Coagulation_factor_XIIa Coagulation factor XIIa], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.38 3.4.21.38] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xe4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xe4 OCA], [http://pdbe.org/4xe4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xe4 RCSB], [http://www.ebi.ac.uk/pdbsum/4xe4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xe4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xe4 OCA], [http://pdbe.org/4xe4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xe4 RCSB], [http://www.ebi.ac.uk/pdbsum/4xe4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xe4 ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 25: / Line 27: @@
 </StructureSection>
 [[Category: Coagulation factor XIIa]]
+[[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Awford, J]]
 [[Category: Dekker, L V]]

4xe4

From Proteopedia

Revision as of 07:19, 29 May 2019

Coagulation Factor XII protease domain crystal structure

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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