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5nod

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Revision as of 06:05, 12 June 2019

PASTA subunit 4 of Streptococcus pneumoniae STKP crystallized with PEG and succinate

Structural highlights

5nod is a 1 chain structure with sequence from Strr6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	stkP, pkn2, spr1577 (STRR6)
Activity:	Non-specific serine/threonine protein kinase, with EC number 2.7.11.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[STKP_STRR6] Protein kinase involved in signal transduction pathways that regulate various cellular processes. Likely senses intracellular peptidoglycan subunits present in the cell division septa of actively growing cells; thus, intracellular unlinked peptidoglycan may serve as the signal molecules that trigger StkP phosphorylation activity on a set of substrates. Plays a crucial role in the regulation of cell shape and cell division of S.pneumoniae through control of at least DivIVA activity. Identified target substrates that are specifically phosphorylated by StkP in vivo, mainly on threonine residues, are DivIVA and StkP itself. Autophosphorylated StkP is a substrate for the cotranscribed protein phosphatase PhpP (shown in the avirulent strain Rx / Cp1015); PhpP and StkP appear to constitute a functional signaling couple in vivo.^[1] ^[2]

Publication Abstract from PubMed

Eukaryotic-like serine/threonine kinases (eSTKs) with extracellular PASTA repeats are key membrane regulators of bacterial cell division. How PASTA repeats govern eSTK activation and function remains elusive. Using evolution- and structural-guided approaches combined with cell imaging, we disentangle the role of each PASTA repeat of the eSTK StkP from Streptococcus pneumoniae. While the three membrane-proximal PASTA repeats behave as interchangeable modules required for the activation of StkP independently of cell wall binding, they also control the septal cell wall thickness. In contrast, the fourth and membrane-distal PASTA repeat directs StkP localization at the division septum and encompasses a specific motif that is critical for final cell separation through interaction with the cell wall hydrolase LytB. We propose a model in which the extracellular four-PASTA domain of StkP plays a dual function in interconnecting the phosphorylation of StkP endogenous targets along with septal cell wall remodelling to allow cell division of the pneumococcus.

PASTA repeats of the protein kinase StkP interconnect cell constriction and separation of Streptococcus pneumoniae.,Zucchini L, Mercy C, Garcia PS, Cluzel C, Gueguen-Chaignon V, Galisson F, Freton C, Guiral S, Brochier-Armanet C, Gouet P, Grangeasse C Nat Microbiol. 2017 Dec 4. pii: 10.1038/s41564-017-0069-3. doi:, 10.1038/s41564-017-0069-3. PMID:29203882^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fleurie A, Cluzel C, Guiral S, Freton C, Galisson F, Zanella-Cleon I, Di Guilmi AM, Grangeasse C. Mutational dissection of the S/T-kinase StkP reveals crucial roles in cell division of Streptococcus pneumoniae. Mol Microbiol. 2012 Feb;83(4):746-58. doi: 10.1111/j.1365-2958.2011.07962.x. Epub, 2012 Jan 11. PMID:22211696 doi:http://dx.doi.org/10.1111/j.1365-2958.2011.07962.x
↑ Beilharz K, Novakova L, Fadda D, Branny P, Massidda O, Veening JW. Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP. Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):E905-13. doi:, 10.1073/pnas.1119172109. Epub 2012 Mar 19. PMID:22431591 doi:http://dx.doi.org/10.1073/pnas.1119172109
↑ Zucchini L, Mercy C, Garcia PS, Cluzel C, Gueguen-Chaignon V, Galisson F, Freton C, Guiral S, Brochier-Armanet C, Gouet P, Grangeasse C. PASTA repeats of the protein kinase StkP interconnect cell constriction and separation of Streptococcus pneumoniae. Nat Microbiol. 2017 Dec 4. pii: 10.1038/s41564-017-0069-3. doi:, 10.1038/s41564-017-0069-3. PMID:29203882 doi:http://dx.doi.org/10.1038/s41564-017-0069-3

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5nod"

@@ Line 1: / Line 1: @@
 ==PASTA subunit 4 of Streptococcus pneumoniae STKP crystallized with PEG and succinate==
-<StructureSection load='5nod' size='340' side='right' caption='[[5nod]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='5nod' size='340' side='right'caption='[[5nod]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[5nod]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strr6 Strr6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NOD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NOD FirstGlance]. <br>
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Non-specific serine/threonine protein kinase]]
 [[Category: Strr6]]
-[[Category: GALISSON, F]]
+[[Category: Galisson, F]]
-[[Category: GOUET, P]]
+[[Category: Gouet, P]]
-[[Category: GRANGEASSE, C]]
+[[Category: Grangeasse, C]]
-[[Category: GUEGUEN-CHAIGNON, V]]
+[[Category: Gueguen-Chaignon, V]]
-[[Category: ZUCCHINI, L]]
+[[Category: Zucchini, L]]
 [[Category: Extracellular domain]]
 [[Category: Pasta]]

5nod

From Proteopedia

Revision as of 06:05, 12 June 2019

PASTA subunit 4 of Streptococcus pneumoniae STKP crystallized with PEG and succinate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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