DNA methyltransferase

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*'''Methylcytosine DNMT''' methylates the 5 position of cytosine. <ref>PMID:8127644</ref><br />
*'''Methylcytosine DNMT''' methylates the 5 position of cytosine. <ref>PMID:8127644</ref><br />
*'''Methyladenine DNMT''' methylates the 6 position of adenine. <ref>PMID:12732637</ref><br />
*'''Methyladenine DNMT''' methylates the 6 position of adenine. <ref>PMID:12732637</ref><br />
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*'''HhaI DNA methyltransferase''' (HDMT), a DNMT from ''Haemophilus haemolyticus'' recognizes the 5’-GCGC-3’ DNA sequence and methylates the first cytosine to convert it to 5-methylcytosine. The SAM serves as the methyl donor in the reaction and is converted to to S-adenosyl-L-homocysteine (SAH) by the reaction<ref>PMID:12139442</ref>.
== Relevance ==
== Relevance ==
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== Structural highlights ==
== Structural highlights ==
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The <scene name='46/467277/Cv/6'>active site of methyladenine DNMT contains the methyl donor SAM</scene>.
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The <scene name='46/467277/Cv/8'>active site of methyladenine DNMT contains the methyl donor SAM</scene>.
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</StructureSection>
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== 3D Structures of DNA methyltransferase ==
== 3D Structures of DNA methyltransferase ==
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[[DNA methyltransferase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Methylguanine DNMT
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**[[1sfe]] – O6-methylguanine DNMT C terminal – ''Escherichia coli''
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**[[1qnt]], [[1eh6]], [[3llr]] - hO6-methylguanine DNMT – human
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**[[1t38]] - hO6-methylguanine DNMT (mutant) + DNA
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**[[1t39]], [[1yfh]] - hO6-methylguanine DNMT + DNA
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**[[1eh7]] - hO6-methylguanine DNMT methylated
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**[[1eh8]] - hO6-methylguanine DNMT benzylated<br />
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**[[1mgt]] - O6-methylguanine DNMT – ''Thermococcus kodakarensis''<br />
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**[[1wrj]] - O6-methylguanine DNMT - ''Sulfolobus tokodaii''
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**[[2g7h]] - O6-methylguanine DNMT – ''Methanocaldococcus jannaschii''
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**[[2kif]], [[2kim]] - O6-methylguanine DNMT – ''Vibrio parahaemolyticus'' - NMR<br />
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**[[4bhb]], [[4bhc]] - O6-methylguanine DNMT (mutant) – ''Mycobacterium tuberculosis''<br />
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*Methylcytosine DNMT
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**[[2qrv]] - h-cytosine DNMT
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**[[3epz]] - h-cytosine DNMT replecation foci-targeting sequence
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**[[3flg]] - h-cytosine DNMT ADD domain
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**[[3a1a]] - h-cytosine DNMT PWWP domain
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**[[1g55]] - h-cytosine DNMT2 (mutant)<br />
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**[[3qkj]] - h-cytosine DNMT 3β<br />
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**[[3av4]], [[1khc]] - m-cytosine DNMT – mouse
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**[[2uyc]], [[2uyh]], [[2uz4]] - Hh-cytosine DNMT (mutant) + DNA + SAH
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**[[3a1b]] - h-cytosine DNMT ADD domain + histone H3
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**[[3pt6]], [[3pt9]], [[4da4]] – m-cytosine DNMT + DNA
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**[[3av5]] - m-cytosine DNMT + SAH
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**[[3av6]] - m-cytosine DNMT + SAM
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**[[3swr]] - h-cytosine DNMT + sinefungin
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**[[1boo]] - cytosine DNMT (mutant) – ''Proteus vulgaris''<br />
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**[[3qv2]] - cytosine DNMT + SAH – ''Entamoeba histolytica''<br />
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**[[2uyc]], [[2uyh]], [[2uz4]], [[1fjx]] – Hh-cytosine DNMT (mutant) + DNA + SAH - ''Haemophilus haemolyticus''<br />
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**[[2c7o]], [[2c7p]], [[2c7q]], [[2c7r]], [[3pta]] – Hh-cytosine DNMT + DNA + S-adenonosyl cysteine<br />
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**[[1hmy]] – Hh-cytosine Haeiii DNMT + SAM
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**[[1m0e]] - Hh-cytosine DNMT (mutant) + DNA + inhibitor
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**[[2i9k]] - Hh-cytosine DNMT (mutant) + DNA
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**[[2hr1]] - Hh-cytosine DNMT + DNA + ADOHCY
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**[[2z6q]], [[2z6u]], [[2zcj]] - Hh-cytosine DNMT (mutant) + DNA + ADOHCY
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**[[1svu]] - Hh-cytosine DNMT (mutant)<br />
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**[[4fsx]] – c-cytosine DNMT + SAH – corn<br />
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**[[4ft2]], [[4ft4]] – c-cytosine DNMT + histone H3 peptide<br />
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**[[4h0n]] - cytosine DNMT (mutant) – ''Spodoptera frugiperda''
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*Methyladenine DNMT
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**[[1nw5]] – RsN6-adenine DNMT + SAM – ''Rhodobacter sphaeroides''
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**[[1nw6]] - RsN6-adenine DNMT + sinefungin<br />
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**[[1nw7]] - RsN6-adenine DNMT + SAH<br />
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**[[1nw8]] - RsN6-adenine DNMT (mutant)
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**[[2ibs]], [[2ibt]], [[2ih2]], [[2ih4]], [[2ih5]], [[2np6]], [[2np7]], [[1g38]] - Ta-adenine DNMT Taqi + DNA + cofactor analog – ''Thermus aquaticus''<br />
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**[[1aqi]] – Ta-adenine DNMT Taqi + SAH<br />
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**[[1aqj]] – Ta-adenine DNMT Taqi + sinefugin<br />
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**[[2adm]] - Ta-adenine DNMT Taqi + SAM<br />
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**[[1g60]] - adenine DNMT + SAM – ''Moraxella bovis''
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}}
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== References ==
== References ==

Current revision

Adenine DNMT complex with SAM and Cl- ion (PDB code 1nw5).

Drag the structure with the mouse to rotate

References

  1. ↑ Kaina B, Christmann M, Naumann S, Roos WP. MGMT: key node in the battle against genotoxicity, carcinogenicity and apoptosis induced by alkylating agents. DNA Repair (Amst). 2007 Aug 1;6(8):1079-99. Epub 2007 May 7. PMID:17485253 doi:http://dx.doi.org/10.1016/j.dnarep.2007.03.008
  2. ↑ Kumar S, Cheng X, Klimasauskas S, Mi S, Posfai J, Roberts RJ, Wilson GG. The DNA (cytosine-5) methyltransferases. Nucleic Acids Res. 1994 Jan 11;22(1):1-10. PMID:8127644
  3. ↑ Thomas CB, Scavetta RD, Gumport RI, Churchill ME. Structures of liganded and unliganded RsrI N6-adenine DNA methyltransferase: a distinct orientation for active cofactor binding. J Biol Chem. 2003 Jul 11;278(28):26094-101. Epub 2003 May 4. PMID:12732637 doi:http://dx.doi.org/10.1074/jbc.M303751200
  4. ↑ Sankpal UT, Rao DN. Structure, function, and mechanism of HhaI DNA methyltransferases. Crit Rev Biochem Mol Biol. 2002;37(3):167-97. PMID:12139442 doi:http://dx.doi.org/10.1080/10409230290771492

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Michal Harel, Alexander Berchansky

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