Elastase

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[[Image:3ba0.png|left|200px|thumb|Crystal structure of full-length human Elastase (Metalloproteinase-12), [[3ba0]]]]
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<StructureSection load='1eai' size='350' side='right' caption='Pig elastase (cyan, green) complex with chymotrypsin/elastase isoinhibitor (magenta, yellow) (PDB code [[1eai]]).' scene='41/411401/Cv/3'>
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{{STRUCTURE_3ba0| PDB=3ba0 | SIZE=300| SCENE=Elastase/Cv/2 |right|CAPTION=Human Elastase (Metalloproteinase-12), [[3ba0]] }}
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[[Elastase]] (ELA) is a chymotrypsin-like protease which breaks down proteins. The ELAs are numbered according to their genes. ELA2 (or PMN-ELA) is secreted by neutrophils during inflammation. The images at the left and at the right correspond to one representative elastase structure, ''i.e.'' the human Matrix Metalloproteinase-12 (MMP12), which is macrophage ELA ([[3ba0]]). Calcium ions are shown as green balls, zinc ions are grey balls and acetohydroxamic acid is shown as space filling object. For additional details see [[Serine Proteases]].
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[[Elastase]] (ELA) is a chymotrypsin-like serine protease which breaks down proteins. The ELAs are numbered according to their genes. '''Panceatic ELA''' cleaves proteins at the carboxyl-terminal side of small neutral amino acids. It cleaves Ala-Ala and Ala-Gly bonds. <ref>PMID:12059069</ref> For additional details see <br />
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*[[Protease]]<br />
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*[[Serine Proteases]].
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{{TOC limit|limit=2}}
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== Relevance ==
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==3D structures of elastase==
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'''ELA1''' in the pancreas correlates well with exocrine pancreatic functioning and is used as a function test for patients with pancreatitis. '''ELA2''' (or PMN-ELA) is secreted by neutrophils during inflammation.
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''Update June 2011''
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== Disease ==
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== MMP12 ==
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Pulmonary emphysema is caused by the destruction of elastic fibre by elastase. Mutations in ELA2 are linked to the genetic disorder cyclic hematopoeiesis. Neutrophil elastase is involved in the blistering in bullose pemphigoid.
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== Structural highlights ==
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[[3lka]] - hMMP12 catalytic domain+hydroxamic acid+paramethoxy-sulfonyl amide – human<br />
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<scene name='41/411401/Cv/8'>Pig elastase</scene> (cyan, green) complex with <scene name='41/411401/Cv/9'>chymotrypsin/elastase isoinhibitor</scene> (magenta, yellow) (PDB code [[1eai]]). ELA <scene name='41/411401/Cv/10'>catalytic triad is Ser-His-Asp</scene>.<ref>PMID:7922044</ref>
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[[3ba0]], [[1jk3]] – hMMP12<br />
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[[3ehx]], [[3ehy]], [[1utt]], [[1utz]], [[1ros]] - hMMP12 catalytic domain+inhibitor<br />
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[[2oxu]] - hMMP12 catalytic domain<br />
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[[2oxw]], [[2oxz]] - hMMP12 catalytic domain+peptide<br />
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[[2hu6]] - hMMP12 catalytic domain+bicyclic inhibitor<br />
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[[1rmz]] - hMMP12 catalytic domain+ inhibitor NNGH<br />
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[[1y93]] - hMMP12 catalytic domain+acetohydroxamic acid<br />
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[[1jiz]] – hMMP12+ hydroxamic acid<br />
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[[1os2]] - hMMP12 (mutant)+hydroxamic acid<br />
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[[1os9]] - hMMP12 (mutant)<br />
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[[3f15]], [[3f16]], [[3f17]], [[3f18]], [[3f19]], [[3f1a]], [[3n2u]], [[3n2v]], [[3nx7]] - hMMP12 catalytic domain+sulfonamide inhibitor<br />
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[[2w0d]] - hMMP12 catalytic domain (mutant)<br />
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[[2krj]], [[2k9c]] – hMMP12 fraction – NMR<br />
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[[2jxy]] - hMMP12 hemopexin-like domain – NMR<br />
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[[2k2g]] - hMMP12 catalytic domain+inhibitor – NMR<br />
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[[2poj]] - hMMP12 catalytic domain – NMR<br />
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[[2z2d]] - hMMP12 catalytic domain+butanoic acid – NMR<br />
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[[1ycm]], [[1z3j]] – hMMP12 (mutant)+hydroxamic acid derivative - NMR<br />
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[[3lk8]] - hMMP12 catalytic domain (mutant)+hydroxamic acid derivative<br />
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[[2wo8]], [[2wo9]], [[2woa]] – hMMP12 fraction+hydroxy carboxylic acid<br />
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[[3lik]], [[3lil]], [[3lir]], [[3ljg]] – hMMP12 catalytic domain (mutant)+chelating inhibitor
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== MMP16 ==
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==3D structures of elastase==
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[[Elastase 3D structures]]
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[[1rm8]] – hMMP16+batimastat
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== ELA ==
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[[3mty]], [[3mu0]], [[3mu1]], [[3mu4]], [[3mu5]], [[3mu8]], [[3mnb]], [[3mnc]], [[3mns]], [[3mnx]], [[3mo3]], [[3mo6]], [[3mo9]], [[3moc]], [[3e3t]], [[2v0b]], [[2g4t]], [[2g4u]], [[2de8]], [[2blo]], [[2blq]], [[2a7c]], [[2a7j]], [[1gvk]], [[1qnj]], [[1lvy]], [[3est]], [[1est]], [[2oqu]], [[3odd]], [[3odf]] – pELA1 – pig<br />
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[[3hgn]] – pELA1+peptidyl inhibitor – neutron<br />
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[[3hgp]], [[2cv3]], [[2bdb]], [[2bcd]], [[2h1u]], [[1mmj]], [[1h9l]], [[1nes]], [[4est]], [[5est]], [[8est]], [[7est]], [[6est]] - pELA1+peptidyl inhibitor<br />
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[[1fzz]] – pELA1+ONO-6818<br />
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[[1qr3]], [[1qgf]], [[1bru]], [[1hv7]], [[1btu]] – pELA1+cyclic inhibitor<br />
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[[1e34]], [[1e35]], [[1e36]], [[1e37]], [[1e38]] – pELA1-gamma-lactam<br />
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[[1eai]] – pELA1+Ascaris inhibitor<br />
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[[2d26]] – pELA1+α-1-antitrypsin<br />
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[[1fle]] – pELA1+elafin<br />
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[[1b0e]], [[1b0f]] – pELA1+MDL<br />
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[[2v35]] – pELA1+JM54<br />
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[[2iot]] – pELA1+clavulanic acid<br />
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[[2de9]] – pELA1+tris<br />
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[[1uvo]], [[1uvp]], [[1lka]], [[1lkb]] – pELA1+ions<br />
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[[1gwa]] – pELA1+I3<br />
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[[1uo6]], [[1c1m]] – pELA1+Xe<br />
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[[1l0z]], [[1l1g]] - pELA1+Xe+Br<br />
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[[2bb4]], [[2bd2]], [[2bd3]], [[2bd4]], [[2bd5]], [[2bd7]], [[2bd8]], [[2bd9]], [[1hax]], [[1hay]], [[1haz]], [[1hb0]], [[1qix]] – pELA1+beta-casomorphin-7<br />
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[[2bda]] – pELA1+N-acetyl-NPI<br />
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[[1mcv]] – pELA1+squash inhibitor<br />
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[[1bma]] – pELA1+aminimide<br />
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[[1elf]], [[1elg]] – pELA1+peptidyl hydroxylamine<br />
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[[1eld]], [[1ele]], [[1elb]], [[1ela]], [[1elc]], [[2est]] – pELA1+anilide inhibitor<br />
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[[1esa]], [[1esb]] – pELA1 intermediate<br />
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[[1eas]], [[1eat]], [[1eau]] – pELA1+pyridine inhibitor<br />
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[[1inc]] – pELA1+benzoxazinone<br />
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[[1jim]], [[9est]], [[8est]] – pELA1+coumarin derivative<br />
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[[2fo9]], [[2foa]], [[2fob]], [[2foc]], [[2fod]], [[2foe]], [[2fof]], [[2fog]], [[2foh]] – pELA1+solvent<br />
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[[3dbk]] – PaELA+phophoramidon – Pseudomonas aeruginosa<br />
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[[1u4g]] – PaELA+HPI<br />
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[[1ezm]] - PaELA<br />
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[[1elt]] – ELA - salmon<br />
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== ELA2 ==
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[[3q76]] – hELA2<br />
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[[3q77]] – hELA2+dihydropyrimidone inhibitor<br />
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[[2z7f]] – hELA2 peptidase domain +antileukoproteinase<br />
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[[2rg3]] – hELA2+sulfonic inhibitor<br />
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[[1h1b]], [[1ppg]], [[1hne]] – hELA2+inhibitor<br />
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[[1ppf]] – hELA2+ovomucoid<br />
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[[1b0f]] – hELA2+MDL101146<br />
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</StructureSection>
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Pig elastase (cyan, green) complex with chymotrypsin/elastase isoinhibitor (magenta, yellow) (PDB code 1eai).

Drag the structure with the mouse to rotate

References

  1. ↑ Dominici R, Franzini C. Fecal elastase-1 as a test for pancreatic function: a review. Clin Chem Lab Med. 2002 Apr;40(4):325-32. PMID:12059069 doi:http://dx.doi.org/10.1515/CCLM.2002.051
  2. ↑ Huang K, Strynadka NC, Bernard VD, Peanasky RJ, James MN. The molecular structure of the complex of Ascaris chymotrypsin/elastase inhibitor with porcine elastase. Structure. 1994 Jul 15;2(7):679-89. PMID:7922044

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Guido Hansen, Jaime Prilusky

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