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Exoenzyme

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<StructureSection load='2c8c' size='340' side='right' caption='Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code [[2c8c]]).' scene=''>
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<StructureSection load='2c8c' size='400' side='right' caption='Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code [[2c8c]]).' scene='59/593306/Cv/8'>
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'''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. '''Exo C3''' is a ''Clostridium butulinum'' toxin which binds NAD, splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. '''Exo S''' and '''Exo T''' are secreted by ''Pseudomonas aeruginosa'' and has GTPase-activating activity as well as ADP-ribosyltransferase activity<ref>PMID:11114250</ref> .
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'''Exoenzymes''' (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. '''Exo C3''' is a ''Clostridium butulinum'' toxin which binds [[NAD]], splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. '''Exo S''' and '''Exo T''' are secreted by ''Pseudomonas aeruginosa'' and has GTPase-activating activity as well as ADP-ribosyltransferase activity<ref>PMID:11114250</ref> .
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</StructureSection>
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<scene name='59/593306/Cv/6'>NAD binding site</scene>.
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<scene name='59/593306/Cv/7'>ADP binding site</scene> in exoenzyme C3 catalytic domain (PDB code [[2c8c]]). <ref>PMID:18369192</ref>
==3D structures of exoenzyme==
==3D structures of exoenzyme==
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[[Exoenzyme 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Exoenzyme C3
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**[[1g24]] – CbExo residues 8-218 – ''Clostridium butulinum''<br />
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**[[1uzi]], [[1r45]], [[1r4b]], [[2c89]] – CbExo catalytic domain <br />
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**[[1gze]], [[2c8b]], [[2c8d]], [[2c8e]], [[2c8g]] – CbExo catalytic domain (mutant)<br />
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**[[3bw8]] - Exo catalytic domain - ''Clostridium limosum''<br />
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*Exoenzyme C3 complexes
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**[[1gzf]] – CbExo catalytic domain + pyridinium derivative + ADP + NAD<br />
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**[[2bov]] – CbExo + GDP + Ral-A GTPase<br />
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**[[2a78]] – CbExo catalytic domain + GDP + Ral-A GTPase<br />
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**[[2a9k]] – CbExo catalytic domain+ GDP + Ral-A GTPase + NAD<br />
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**[[2c8a]] – CbExo catalytic domain + nicotinamide<br />
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**[[2c8c]] – CbExo catalytic domain (mutant) + ADP + NAD<br />
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**[[2c8f]], [[2c8h]] – CbExo catalytic domain (mutant) + NAD<br />
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*Exoenzyme S
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**[[1r4t]] – PaExo GAP domain – ''Pseudomonas aeruginosa'' - NMR<br />
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**[[1he1]] – PaExo GAP domain + Ras-like protein + GDP <br />
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**[[1he9]] – PaExo GAP domain + Ras-like protein <br />
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*Exoenzyme T
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**[[4jmf]] – PaExo residues 28-77 + chaperone<br />
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}}
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== References ==
== References ==

Current revision

Structure of exoenzyme C3 catalytic domain complex with ADP and NAD (PDB code 2c8c).

Drag the structure with the mouse to rotate

References

  1. ↑ Han S, Arvai AS, Clancy SB, Tainer JA. Crystal structure and novel recognition motif of rho ADP-ribosylating C3 exoenzyme from Clostridium botulinum: structural insights for recognition specificity and catalysis. J Mol Biol. 2001 Jan 5;305(1):95-107. PMID:11114250 doi:10.1006/jmbi.2000.4292
  2. ↑ Menetrey J, Flatau G, Boquet P, Menez A, Stura EA. Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes. Protein Sci. 2008 May;17(5):878-86. Epub 2008 Mar 27. PMID:18369192 doi:10.1110/ps.073398508

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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