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Met repressor
From Proteopedia
(Difference between revisions)
(New page: EcMR (mutant) complex with corepressor SAM and DNA 2eyi {{STRUCTURE_1mjo| PDB=1mjo | SIZE=400| SCENE= |right|CAPTION=EcMR (mutant) complex with...) |
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| - | + | <StructureSection load='1mjo' size='450' side='right' scene='53/536702/Cv/1' caption='EcMR (mutant) complex with corepressor SAM, Ca+2 ion (green) and DNA [[1mjo]]'> | |
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| + | == Function == | ||
| + | '''Met repressor''' (MR) interacts with the DNA operator (Met box) repressing methionine synthesis. The co-repressor of MR is S-adenosyl methionine (SAM) which is a product of methionine synthesis. Binding of SAM to MR increases the repressor affinity to the DNA thus halting methionine synthesis<ref>PMID:8092669</ref>. For additional details see<br /> | ||
| + | [[Taylor met repressor]]<br /> | ||
| + | [[Joe Granger Methionine Repressor: Escherichia coli]]. | ||
| - | + | == Structural highlights == | |
| - | + | <scene name='53/536702/Cv/7'>EcMR (mutant) with corepressor SAM, Ca+2 ion and DNA</scene>. <scene name='53/536702/Cv/8'>SAM binds to MR in a hydrophobic pocket between 2 monomers</scene><ref>PMID:10986458</ref>. Water molecules are shown as red spheres. | |
| - | + | </StructureSection> | |
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== 3D Structures of Met repressor == | == 3D Structures of Met repressor == | ||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | [[1cmb]] – EcMR – Escherichia coli<br /> | + | [[1cmb]] – EcMR – ''Escherichia coli''<br /> |
[[1mjk]] – EcMR (mutant)<br /> | [[1mjk]] – EcMR (mutant)<br /> | ||
[[1cma]] – EcMR + DNA<br /> | [[1cma]] – EcMR + DNA<br /> | ||
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[[1mjl]], [[1mjp]] – EcMR (mutant) + SAM<br /> | [[1mjl]], [[1mjp]] – EcMR (mutant) + SAM<br /> | ||
[[1mj2]], [[1mjo]], [[1mjq]] – EcMR (mutant) + SAM + DNA<br /> | [[1mj2]], [[1mjo]], [[1mjq]] – EcMR (mutant) + SAM + DNA<br /> | ||
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| + | == References == | ||
| + | <references/> | ||
| + | [[Category:Topic Page]] | ||
Current revision
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3D Structures of Met repressor
Updated on 10-July-2019
1cmb – EcMR – Escherichia coli
1mjk – EcMR (mutant)
1cma – EcMR + DNA
1mjm – EcMR (mutant) + DNA
1cmc – EcMR + SAM
1mjl, 1mjp – EcMR (mutant) + SAM
1mj2, 1mjo, 1mjq – EcMR (mutant) + SAM + DNA
References
- ↑ Somers WS, Rafferty JB, Phillips K, Strathdee S, He YY, McNally T, Manfield I, Navratil O, Old IG, Saint-Girons I, et al.. The Met repressor-operator complex: DNA recognition by beta-strands. Ann N Y Acad Sci. 1994 Jul 29;726:105-17. PMID:8092669
- ↑ Garvie CW, Phillips SE. Direct and indirect readout in mutant Met repressor-operator complexes. Structure. 2000 Sep 15;8(9):905-14. PMID:10986458
