Met repressor

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<StructureSection load='1mjo' size='450' side='right' scene='53/536702/Cv/1' caption='EcMR (mutant) complex with corepressor SAM, Ca+2 ion (green) and DNA [[1mjo]]'>
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[[Image:1mjo.png|left|200px|thumb|EcMR (mutant) complex with corepressor SAM and DNA [[2eyi]]]]
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{{STRUCTURE_1mjo| PDB=1mjo | SIZE=400| SCENE= |right|CAPTION=EcMR (mutant) complex with corepressor SAM Ca+2 ion and DNA [[1mjo]] }}
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== Function ==
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'''Met repressor''' (MR) interacts with the DNA operator (Met box) repressing methionine synthesis. The co-repressor of MR is S-adenosyl methionine (SAM) which is a product of methionine synthesis. Binding of SAM to MR increases the repressor affinity to the DNA thus halting methionine synthesis<ref>PMID:8092669</ref>. For additional details see<br />
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[[Taylor met repressor]]<br />
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[[Joe Granger Methionine Repressor: Escherichia coli]].
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== Structural highlights ==
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<scene name='53/536702/Cv/7'>EcMR (mutant) with corepressor SAM, Ca+2 ion and DNA</scene>. <scene name='53/536702/Cv/8'>SAM binds to MR in a hydrophobic pocket between 2 monomers</scene><ref>PMID:10986458</ref>. Water molecules are shown as red spheres.
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</StructureSection>
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'''Met repressor''' (MR) interacts with the DNA operator (Met box) repressing methionine synthesis. The corepressor of MR is S-adenosyl methionine (SAM) which is a product of methionine synthesis. Binding of SAM to MR increases the repressor affinity to the DNA thus halting methionine synthesis. For additional details see [[Joe Granger Methionine Repressor: Escherichia coli]].
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== 3D Structures of Met repressor ==
== 3D Structures of Met repressor ==
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[[1mjl]], [[1mjp]] – EcMR (mutant) + SAM<br />
[[1mjl]], [[1mjp]] – EcMR (mutant) + SAM<br />
[[1mj2]], [[1mjo]], [[1mjq]] – EcMR (mutant) + SAM + DNA<br />
[[1mj2]], [[1mjo]], [[1mjq]] – EcMR (mutant) + SAM + DNA<br />
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

EcMR (mutant) complex with corepressor SAM, Ca+2 ion (green) and DNA 1mjo

Drag the structure with the mouse to rotate

3D Structures of Met repressor

Updated on 10-July-2019

1cmb – EcMR – Escherichia coli
1mjk – EcMR (mutant)
1cma – EcMR + DNA
1mjm – EcMR (mutant) + DNA
1cmc – EcMR + SAM
1mjl, 1mjp – EcMR (mutant) + SAM
1mj2, 1mjo, 1mjq – EcMR (mutant) + SAM + DNA

References

  1. ↑ Somers WS, Rafferty JB, Phillips K, Strathdee S, He YY, McNally T, Manfield I, Navratil O, Old IG, Saint-Girons I, et al.. The Met repressor-operator complex: DNA recognition by beta-strands. Ann N Y Acad Sci. 1994 Jul 29;726:105-17. PMID:8092669
  2. ↑ Garvie CW, Phillips SE. Direct and indirect readout in mutant Met repressor-operator complexes. Structure. 2000 Sep 15;8(9):905-14. PMID:10986458

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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