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6jcg

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'''Unreleased structure'''
 
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The entry 6jcg is ON HOLD until Paper Publication
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==Room temperature structure of HIV-1 Integrase catalytic core domain by serial femtosecond crystallography.==
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<StructureSection load='6jcg' size='340' side='right'caption='[[6jcg]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6jcg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JCG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jcg OCA], [http://pdbe.org/6jcg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jcg RCSB], [http://www.ebi.ac.uk/pdbsum/6jcg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jcg ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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HIV-1 integrase (HIV-1 IN) is an enzyme produced by the HIV-1 virus that integrates genetic material of the virus into the DNA of infected human cells. HIV-1 IN acts as a key component of the Retroviral Pre-Integration Complex (PIC). Protein dynamics could play an important role during the catalysis of HIV-1 IN; however, this process has not yet been fully elucidated. X-ray free electron laser (XFEL) together with nuclear magnetic resonance (NMR) could provide information regarding the dynamics during this catalysis reaction. Here, we report the non-cryogenic crystal structure of HIV-1 IN catalytic core domain at 2.5 A using microcrystals in XFELs. Compared to the cryogenic structure at 2.1 A using conventional synchrotron crystallography, there was a good agreement between the two structures, except for a catalytic triad formed by Asp64, Asp116, and Glu152 (DDE) and the lens epithelium-derived growth factor binding sites. The helix III region of the 140-153 residues near the active site and the DDE triad show a higher dynamic profile in the non-cryogenic structure, which is comparable to dynamics data obtained from NMR spectroscopy in solution state.
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Authors:
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Non-Cryogenic Structure and Dynamics of HIV-1 Integrase Catalytic Core Domain by X-ray Free-Electron Lasers.,Park JH, Yun JH, Shi Y, Han J, Li X, Jin Z, Kim T, Park J, Park S, Liu H, Lee W Int J Mol Sci. 2019 Apr 20;20(8). pii: ijms20081943. doi: 10.3390/ijms20081943. PMID:31010024<ref>PMID:31010024</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 6jcg" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Han, J]]
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[[Category: Kim, T H]]
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[[Category: Li, X]]
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[[Category: Park, J H]]
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[[Category: Shi, Y]]
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[[Category: Yun, J H]]
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[[Category: Hiv]]
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[[Category: Hiv-1]]
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[[Category: Integrase]]
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[[Category: Room temperature]]
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[[Category: Viral protein]]

Revision as of 10:40, 17 July 2019

Room temperature structure of HIV-1 Integrase catalytic core domain by serial femtosecond crystallography.

PDB ID 6jcg

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