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1di1

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Revision as of 14:02, 16 October 2019

CRYSTAL STRUCTURE OF ARISTOLOCHENE SYNTHASE FROM PENICILLIUM ROQUEFORTI

Structural highlights

1di1 is a 2 chain structure with sequence from Atcc 10110. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1dgp, 1f1l, 1f1k, 1f1n, 1f1p
Activity:	Aristolochene synthase, with EC number 4.2.3.9
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ARIS_PENRO] Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic sesquiterpene aristolochene. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce aristolochene. Aristolochene is the likely parent compound for a number of sesquiterpenoid toxins produced by filamentous fungi.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The 2.5-A resolution crystal structure of recombinant aristolochene synthase from the blue cheese mold, Penicillium roqueforti, is the first of a fungal terpenoid cyclase. The structure of the enzyme reveals active site features that participate in the cyclization of the universal sesquiterpene cyclase substrate, farnesyl diphosphate, to form the bicyclic hydrocarbon aristolochene. Metal-triggered carbocation formation initiates the cyclization cascade, which proceeds through multiple complex intermediates to yield one exclusive structural and stereochemical isomer of aristolochene. Structural homology of this fungal cyclase with plant and bacterial terpenoid cyclases, despite minimal amino acid sequence identity, suggests divergence from a common, primordial ancestor in the evolution of terpene biosynthesis.

Crystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti.,Caruthers JM, Kang I, Rynkiewicz MJ, Cane DE, Christianson DW J Biol Chem. 2000 Aug 18;275(33):25533-9. PMID:10825154^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Proctor RH, Hohn TM. Aristolochene synthase. Isolation, characterization, and bacterial expression of a sesquiterpenoid biosynthetic gene (Ari1) from Penicillium roqueforti. J Biol Chem. 1993 Feb 25;268(6):4543-8. PMID:8440737
↑ Felicetti B, Cane DE. Aristolochene synthase: mechanistic analysis of active site residues by site-directed mutagenesis. J Am Chem Soc. 2004 Jun 16;126(23):7212-21. PMID:15186158 doi:10.1021/ja0499593
↑ Caruthers JM, Kang I, Rynkiewicz MJ, Cane DE, Christianson DW. Crystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti. J Biol Chem. 2000 Aug 18;275(33):25533-9. PMID:10825154 doi:10.1074/jbc.M000433200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1di1"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF ARISTOLOCHENE SYNTHASE FROM PENICILLIUM ROQUEFORTI==
-<StructureSection load='1di1' size='340' side='right' caption='[[1di1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='1di1' size='340' side='right'caption='[[1di1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1di1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10110 Atcc 10110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DI1 FirstGlance]. <br>
@@ Line 35: / Line 35: @@
 [[Category: Aristolochene synthase]]
 [[Category: Atcc 10110]]
+[[Category: Large Structures]]
 [[Category: Cane, D E]]
 [[Category: Caruthers, J M]]

1di1

From Proteopedia

Revision as of 14:02, 16 October 2019

CRYSTAL STRUCTURE OF ARISTOLOCHENE SYNTHASE FROM PENICILLIUM ROQUEFORTI

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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