This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6cp9
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6cp9 is ON HOLD Authors: Michalska, K., Stols, L., Eschenfeldt, W., Hayes, C.S., Goulding, C.W., Joachimiak, A., Midwest Center for Structural Genom...) |
|||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Contact-dependent growth inhibition toxin - immunity protein complex from Klebsiella pneumoniae 342== | |
| + | <StructureSection load='6cp9' size='340' side='right'caption='[[6cp9]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6cp9]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Klep3 Klep3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CP9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CP9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KPK_4110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=507522 KLEP3])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cp9 OCA], [http://pdbe.org/6cp9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cp9 RCSB], [http://www.ebi.ac.uk/pdbsum/6cp9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cp9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Contact-dependent growth inhibition (CDI) is a form of interbacterial competition mediated by CdiB-CdiA two-partner secretion systems. CdiA effector proteins carry polymorphic C-terminal toxin domains (CdiA-CT), which are neutralized by specific CdiI immunity proteins to prevent self-inhibition. Here, we present the crystal structures of CdiA-CTCdiI complexes from Klebsiella pneumoniae 342 and Escherichia coli 3006. The toxins adopt related folds that resemble the ribonuclease domain of colicin D, and both are isoacceptor-specific tRNases that cleave the acceptor stem of deacylated tRNAGAU(Ile). Although the toxins are similar in structure and substrate specificity, CdiA-CT(Kp342) activity requires translation factors EF-Tu and EF-Ts, whereas CdiA-CT(EC3006) is intrinsically active. Furthermore, the corresponding immunity proteins are unrelated in sequence and structure. CdiI(Kp342) forms a dimeric beta sandwich, whereas CdiI(EC3006) is an alpha-solenoid monomer. Given that toxin-immunity genes co-evolve as linked pairs, these observations suggest that the similarities in toxin structure and activity reflect functional convergence. | ||
| - | + | Convergent Evolution of the Barnase/EndoU/Colicin/RelE (BECR) Fold in Antibacterial tRNase Toxins.,Gucinski GC, Michalska K, Garza-Sanchez F, Eschenfeldt WH, Stols L, Nguyen JY, Goulding CW, Joachimiak A, Hayes CS Structure. 2019 Sep 6. pii: S0969-2126(19)30281-3. doi:, 10.1016/j.str.2019.08.010. PMID:31515004<ref>PMID:31515004</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6cp9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Klep3]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Eschenfeldt, W]] | ||
| + | [[Category: Goulding, C W]] | ||
| + | [[Category: Hayes, C S]] | ||
| + | [[Category: Joachimiak, A]] | ||
| + | [[Category: Structural genomic]] | ||
[[Category: Michalska, K]] | [[Category: Michalska, K]] | ||
| - | [[Category: Goulding, C.W]] | ||
| - | [[Category: Midwest Center For Structural Genomics (Mcsg), Structure-Function Analysis Of Polymorphic Cdi Toxin-Immunity Protein Complexes (Uc4cdi)]] | ||
[[Category: Stols, L]] | [[Category: Stols, L]] | ||
| - | [[Category: | + | [[Category: UC4CDI, Structure-Function Analysis of Polymorphic CDI Toxin-Immunity Protein Complexes]] |
| - | [[Category: | + | [[Category: Mcsg]] |
| - | [[Category: | + | [[Category: PSI, Protein structure initiative]] |
| + | [[Category: Psi-biology]] | ||
| + | [[Category: Rnase]] | ||
| + | [[Category: Structure-function analysis of polymorphic cdi toxin-immunity protein complex]] | ||
| + | [[Category: Toxin]] | ||
| + | [[Category: Toxin-antitoxin complex]] | ||
| + | [[Category: Uc4cdi]] | ||
Current revision
Contact-dependent growth inhibition toxin - immunity protein complex from Klebsiella pneumoniae 342
| |||||||||||
Categories: Klep3 | Large Structures | Eschenfeldt, W | Goulding, C W | Hayes, C S | Joachimiak, A | Structural genomic | Michalska, K | Stols, L | UC4CDI, Structure-Function Analysis of Polymorphic CDI Toxin-Immunity Protein Complexes | Mcsg | PSI, Protein structure initiative | Psi-biology | Rnase | Structure-function analysis of polymorphic cdi toxin-immunity protein complex | Toxin | Toxin-antitoxin complex | Uc4cdi
