NAD(P) transhydrogenase

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<StructureSection load='1u28' size='350' side='right' caption='Structure of PTH domains I (grey and green) and III (pink) complex with NAD and NADP (PDB entry [[1u28]])' scene=''>
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<StructureSection load='' size='450' side='right' caption='Structure of PTH domains I (cyan and green) and III (pink) complex with NAD, NADP and glycerol (PDB entry [[1u2d]])' scene='57/571286/Cv/1'>
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'''NAD(P) transhydrogenase''' (PTH) catalyzes the conversion of NADP and NADH to NADPH and NAD. The reaction is coupled with proton translocation across the cell membrane while the enzyme undergoes conformational change. PTH uses FAD as cofactor. PTH is composed of 3 domains.:<br />
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'''NAD(P) transhydrogenase''' (PTH) catalyzes the conversion of NADP and NADH to NADPH and NAD. See [[NAD]] and [[NAD(P)H]]. The reaction is coupled with proton translocation across the cell membrane while the enzyme undergoes conformational change<ref>PMID:12788487</ref>. PTH uses FAD as cofactor.
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== Structural highlights ==
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PTH is composed of 3 domains:<br />
* Domain I binds NAD(+)/NADH.<br />
* Domain I binds NAD(+)/NADH.<br />
* Domain II is a membrane-spanning domain.<br />
* Domain II is a membrane-spanning domain.<br />
* Domain III binds NADP(+)/NADPH.<br />
* Domain III binds NADP(+)/NADPH.<br />
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PTH is composed of 2 subunits. Subunit beta contains domain III and part of II.
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PTH is composed of 2 subunits. Subunit β contains domain III and part of II. The active site of PTH contains <scene name='57/571286/Cv/6'>NAD in domain I</scene> and <scene name='57/571286/Cv/7'>NADP in domain III</scene><ref>PMID:15323555</ref>. Water molecules are shown as red spheres.
==3D structures of NAD(P) transhydrogenase==
==3D structures of NAD(P) transhydrogenase==
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[[NAD(P) transhydrogenase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*NAD(P) transhydrogenase domain I===
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[[1l7d]] – RrPTH domain I – ''Rhodospirillum rubrum'' <br />
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[[1l7e]] – RrPTH domain I + NAD <br />
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[[1x13]] – EcPTH domain I – ''Escherichia coli'' <br />
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[[1x14]], [[1x15]] – EcPTH domain I + NAD <br />
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[[4dio]] – PTH domain I – ''Sinorhizobium meliloti''<br />
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===PTHNAD(P) transhydrogenase domain III===
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[[1e3t]] – RrPTH domain III + NADP - NMR<br />
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[[1pno]] – RrPTH domain III + NADP <br />
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[[1pnq]] – RrPTH domain III + NADPH <br />
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[[1d4o]] – bPTH domain III + NADP - bovine<br />
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[[1djl]], [[1u31]] – hPTH domain III + NADP - human<br />
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[[1pt9]] – hPTH domain III + NADP analog<br />
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===PTHNAD(P) transhydrogenase domain I+III===
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[[1hzz]], [[1u28]], [[1u2d]], [[1xlt]] – RrPTH domain I+III + NADP + NAD<br />
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[[1f8g]] – RrPTH domain I+III + NAD<br />
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[[1nm5]], [[2fr8]], [[2fsv]] – RrPTH domain I+III (mutant) + NADP + NAD<br />
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[[1ptj]], [[1u2g]], [[2frd]], [[2oo5]] – RrPTH domain I+III + NADP + NAD derivative<br />
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[[2oor]] – RrPTH domain I+III + NADP derivative + NAD<br />
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[[2bru]] – EcPTH domain I+III + NADP + NAD - NMR<br />
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[[4j16]], [[4j1t]] – PTH domain I+III + NADP + NAD – ''Thermus thermophilus''<br />
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of PTH domains I (cyan and green) and III (pink) complex with NAD, NADP and glycerol (PDB entry 1u2d)

Drag the structure with the mouse to rotate

References

  1. ↑ Jackson JB. Proton translocation by transhydrogenase. FEBS Lett. 2003 Jun 12;545(1):18-24. PMID:12788487
  2. ↑ Mather OC, Singh A, van Boxel GI, White SA, Jackson JB. Active-site conformational changes associated with hydride transfer in proton-translocating transhydrogenase. Biochemistry. 2004 Aug 31;43(34):10952-64. PMID:15323555 doi:10.1021/bi0497594

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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