Ornithine carbamoyltransferase

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<StructureSection load='4jfr' size='350' side='right' caption='Structure of ornithine carbamoyltransferase complex with carbamoyl phosphate, Mg+2 (small green) and Cl- (large green) ions (PDB entry [[4jfr]])' scene=''>
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<StructureSection load='2i6u' size='450' side='right' caption='Structure of ornithine carbamoyltransferase complex with carbamoyl phosphate, sulfate and the competitive inhibitor norvaline (PDB entry [[2i6u]])' scene='57/573976/Cv/1'>
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== Function ==
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'''Ornithine carbamoyltransferase''' (OTC) catalyzes the conversion of carbamoyl phosphate and ornithine to citrulline and phosphate. OTC is part of the arginine biosynthesis in plants and bacteria and part of the urea cycle in mammals.
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'''Ornithine carbamoyltransferase''' (OTC) catalyzes the conversion of carbamoyl phosphate (CP) and ornithine to citrulline and phosphate. OTC is part of the arginine biosynthesis in plants and bacteria and part of the urea cycle in mammals<ref>PMID:8845761</ref>. Norvaline is a competitive inhibitor of OTC. The '''anabolic OTC''' catalyzes the citrulline synthesis while the '''catabolic OTC''' catalyzes the citrulline phosphorolysis.
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OCT deficiency is a genetic urea cycle disorder.
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==3D structures of ornithine carbamoyltransferase==
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For details see [[Sanbox Reserved 684]].
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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== Disease ==
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[[1a1s]], [[1pvv]] – OTC – ''Pyrococcus furiosus''<br />
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OTC deficiency is a genetic urea cycle disorder<ref>PMID:23278509</ref>.
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[[2ef0]] – OTC – ''Thermus thermophilus''<br />
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[[2p2g]] – MtOTC – ''Mycobacterium tuberculosis''<br />
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== Structural highlights ==
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[[3gd5]] – OTC – ''Gleobacter violaceus''<br />
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The biological assembly of OTC is <scene name='57/573976/Cv/6'>homotrimer</scene>. OTC structure contains an <scene name='57/573976/Cv/7'>N-terminal - CP-binding domain and a C-terminal - ornithine binding domain</scene>. OCT <scene name='57/573976/Cv/8'>active site is situated between the two domains</scene><ref>PMID:18062991</ref>.
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[[3grf]] – OTC – ''Giardia intestinalis''<br />
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[[3tpf]] – OTC – ''Campylobacter jejuni''<br />
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==3D structures of ornithine carbamoyltransferase==
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[[3upd]], [[4kwt]] – VvOTC – ''Vibrio vulnificus''<br />
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[[Ornithine carbamoyltransferase 3D structures]]
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[[4ep1]] – BaOTC (mutant) – ''Bacillus anthracis''<br />
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[[1akm]] – EcOTC – ''Escherichia coli''<br />
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[[1dxh]] – PaOTC – ''Pseudomonas aeruginosa''<br />
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[[1ort]] – PaOTC (mutant) <br />
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[[2w37]] – OTC – ''Lactobacillus hilgardii''<br />
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[[3sds]] – OTC – valley fever fungus<br />
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[[4amu]], [[4anf]] – OTC – ''Mycoplasma penetrans''<br />
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[[4oh7]] – OTC – ''Brucella melitensis''<br />
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===Ornithine carbamoyltransferase complex===
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</StructureSection>
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[[1vlv]] – OTC + phosphate – ''Thermotoga maritima''<br />
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== References ==
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[[2i6u]] – MtOTC + norvaline + carbamoyl phosphate <br />
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<references/>
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[[4f2g]] – OTC + phosphate – ''Burkholderia thailandensis''<br />
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[[4h31]] – VvOTC + norvaline + carbamoyl phosphate<br />
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[[4jfr]] – VvOTC + carbamoyl phosphate<br />
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[[4jhx]] – VvOTC + arginine + carbamoyl phosphate<br />
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[[4jqo]] – VvOTC + citrulline + phosphate<br />
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[[4nf2]] – BaOTC (mutant) + norvaline + carbamoyl phosphate<br />
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[[1c9y]] – hOTC + norvaline + carbamoyl phosphate - human<br />
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[[1ep9]], [[1fvo]] – hOTC + carbamoyl phosphate <br />
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[[1oth]] – hOTC + ornithine derivative<br />
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[[1duv]], [[2otc]] – EcOTC + ornithine derivative<br />
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[[1fb5]] – sOTC + norvaline - sheep<br />
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[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of ornithine carbamoyltransferase complex with carbamoyl phosphate, sulfate and the competitive inhibitor norvaline (PDB entry 2i6u)

Drag the structure with the mouse to rotate

References

  1. ↑ Murata LB, Schachman HK. Structural similarity between ornithine and aspartate transcarbamoylases of Escherichia coli: characterization of the active site and evidence for an interdomain carboxy-terminal helix in ornithine transcarbamoylase. Protein Sci. 1996 Apr;5(4):709-18. PMID:8845761 doi:http://dx.doi.org/10.1002/pro.5560050416
  2. ↑ Storkanova G, Vlaskova H, Chuzhanova N, Zeman J, Stranecky V, Majer F, Peskova K, Luksan O, Jirsa M, Hrebicek M, Dvorakova L. Ornithine carbamoyltransferase deficiency: molecular characterization of 29 families. Clin Genet. 2013 Dec;84(6):552-9. doi: 10.1111/cge.12085. Epub 2013 Feb 7. PMID:23278509 doi:http://dx.doi.org/10.1111/cge.12085
  3. ↑ Sankaranarayanan R, Cherney MM, Cherney LT, Garen CR, Moradian F, James MN. The crystal structures of ornithine carbamoyltransferase from Mycobacterium tuberculosis and its ternary complex with carbamoyl phosphate and L-norvaline reveal the enzyme's catalytic mechanism. J Mol Biol. 2008 Jan 25;375(4):1052-63. Epub 2007 Nov 19. PMID:18062991 doi:10.1016/j.jmb.2007.11.025

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Michal Harel, Alexander Berchansky

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