Sandbox Reserved 1586

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Revision as of 16:12, 18 November 2019

This Sandbox is Reserved from September 14, 2021, through May 31, 2022, for use in the class Introduction to Biochemistry taught by User:John Means at the University of Rio Grande, Rio Grande, OH, USA. This reservation includes 5 reserved sandboxes (Sandbox Reserved 1590 through Sandbox Reserved 1594).

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Leadzyme Structure

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Reference to paper, and to make citation copy this: ^[1] .

1 History
2 Function
3 Disease
4 Relevance
5 Structural highlights

History

Leadzyme was discovered by Uhlenbeck and co-worked in 1992, while they were searching for RNAs that cleaved in the presence of lead ^[2] . The discovery was by in vitro selection, which allows for an isolation and amplification of selected functional molecules. This method has been key in the discovery of numerous RNA and DNA catalysis. Leadzyme, or lead-dependent ribozyme, is among the smallest known catalytic RNAs ^[2] .

Function

Leadzyme is a relatively small catalytic RNA that uses a unique two-step reaction to cleave within the active site ^[3] . In the presence of Pb^2+, it catalyzes the cleavage of the C6-C7 phosphodiester bond ^[4] . This is accomplished via nucleophilic attack ^[5] . The resulting in a 2', 3'-cyclic phosphate and a 5'-hydroxyl terminus ^[6] . The nuclear magnetic resonance spectrospcopy (NMR) and crystal structure of leadzyme has been used to try to betterr understand the cleavage reaction process ^[7] . However, neither data sets revealed the proposed in-line alignment for attack by the 2'-OH nucleophilic group as described experimentally ^[8] . Therefore: it is hypothesized that the structures represent ground states of leadzyme and are not catalytically active ^[9] .

Disease

Relevance

Structural highlights

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References

↑ Hoogstraten CG, Legault P, Pardi A. NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis. J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 doi:S0022-2836(98)92182-9
↑ ^2.0 ^2.1 Hoogstraten CG, Legault P, Pardi A. NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis. J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 doi:S0022-2836(98)92182-9
↑ Qi X, Xia T. Structure, dynamics, and mechanism of the lead-dependent ribozyme. Biomol Concepts. 2011 Aug 1;2(4):305-14. doi: 10.1515/bmc.2011.029. PMID:25962038 doi:http://dx.doi.org/10.1515/bmc.2011.029
↑ Hoogstraten CG, Legault P, Pardi A. NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis. J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 doi:S0022-2836(98)92182-9
↑ Hoogstraten CG, Legault P, Pardi A. NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis. J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 doi:S0022-2836(98)92182-9
↑ Hoogstraten CG, Legault P, Pardi A. NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis. J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 doi:S0022-2836(98)92182-9
↑ Hoogstraten CG, Legault P, Pardi A. NMR solution structure of the lead-dependent ribozyme: evidence for dynamics in RNA catalysis. J Mol Biol. 1998 Nov 27;284(2):337-50. PMID:9813122 doi:S0022-2836(98)92182-9
↑ Qi X, Xia T. Structure, dynamics, and mechanism of the lead-dependent ribozyme. Biomol Concepts. 2011 Aug 1;2(4):305-14. doi: 10.1515/bmc.2011.029. PMID:25962038 doi:http://dx.doi.org/10.1515/bmc.2011.029
↑ Qi X, Xia T. Structure, dynamics, and mechanism of the lead-dependent ribozyme. Biomol Concepts. 2011 Aug 1;2(4):305-14. doi: 10.1515/bmc.2011.029. PMID:25962038 doi:http://dx.doi.org/10.1515/bmc.2011.029

Retrieved from "http://52.214.119.220/wiki/index.php/Sandbox_Reserved_1586"

@@ Line 6: / Line 6: @@
 == History ==
- Leadzyme was discovered by Uhlenbeck and co-worked in 1992, while they were searching for RNAs that cleaved in the presence of lead <ref="red">PMID:9813122</ref> . The discovery was by ''in vitro'' selection, which allows for an isolation and amplification of selected functional molecules. This method has been key in the discovery of numerous RNA and DNA catalysis. Leadzyme, or lead-dependent ribozyme, is among the smallest known catalytic RNAs <ref name=red/> .
+ Leadzyme was discovered by Uhlenbeck and co-worked in 1992, while they were searching for RNAs that cleaved in the presence of lead <ref name="red">PMID:9813122</ref> . The discovery was by ''in vitro'' selection, which allows for an isolation and amplification of selected functional molecules. This method has been key in the discovery of numerous RNA and DNA catalysis. Leadzyme, or lead-dependent ribozyme, is among the smallest known catalytic RNAs <ref name=red/> .
 == Function ==

Sandbox Reserved 1586

From Proteopedia

Revision as of 16:12, 18 November 2019

Leadzyme Structure

Contents

History

Function

Disease

Relevance

Structural highlights

References

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