Pre-mRNA-splicing factor

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:17, 27 November 2019) (edit) (undo)
 
(22 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_3e9l| PDB=3e9l | SIZE=400| SCENE= |right|CAPTION=Human pre-mRNA-splicing factor 8 endonuclease domain complex with Na+ (purple) and Cl- (green) ions, [[3e9l]] }}
+
<StructureSection load='' size='350' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'>
 +
__TOC__
 +
== Function ==
 +
'''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing<ref>PMID:10394358</ref>.
-
'''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing. For details on pre-mRNA-splicing factors see<br />
+
*'''Prp3b''' is part of the U2 small nuclear ribonucleoproteins complex (U2 snRNP)<br />
-
*[[Prp8]]<br />
+
*'''Prp8''' functions as a scaffold which mediates the ordered assembly of spliceosomal proteins and snRNAs. For details see [[Prp8]]<br />
-
*[[Prp24]]<br />
+
*'''Prp18''' and '''Bud31''' are essential for the catalytic step in pre-mRNA splicing process.<br />
-
*[[Prp19]]<br />
+
*'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs. For details see [[Prp24]]<br />
-
*[[Prp40]].
+
*For '''Prp19''' see [[Prp19]]<br />
 +
*For '''Prp20''' see [[Srp20-Human Alternative Splicing Factor]]<br />
 +
*'''Prp31''' functions in forming the U4/U5-U6 tri-snRNP.<br />
 +
*For '''Prp40''' see [[Prp40]].<br />
 +
*'''Prpcwc2''' is a yeast splicing factor which plays a role in the generation of a functional splicing center '''Prp RBM22''' is the human homolog of Prpcwc2.<br />
 +
*'''Prpcwc26''' or '''Bud13''' belongs to the pre-mRNA retention and splicing complex RES.<br />
-
{{TOC limit|limit=2}}
+
== Disease ==
 +
Mutations in Prp8 and Prp31 cause retinitis pigmentosa<ref>PMID:11468273</ref>.
 +
 
 +
== Structural highlights ==
 +
<scene name='46/462200/Cv/3'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.
== 3D Structures of pre-mRNA splicing factors ==
== 3D Structures of pre-mRNA splicing factors ==
 +
[[Pre-mRNA splicing factors 3D structures]]
-
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+
</StructureSection>
-
 
+
-
===Pre-mRNA-splicing factor 2===
+
-
 
+
-
[[3beg]] – hPrp residues 105-219 + Ser/Thr-protein kinase – human<BR />
+
-
[[2o3d]] – hPrp P33 subunit
+
-
 
+
-
===Pre-mRNA-splicing factor 3===
+
-
 
+
-
[[1x4q]] – hPrp PWI domain - NMR<BR />
+
-
 
+
-
===Pre-mRNA-splicing factor 3B===
+
-
 
+
-
[[3lqv]], [[2f9d]], [[2f9j]] – hPrp residues 377-415 + pre-mRNA branch site protein 14<BR />
+
-
[[2fho]] - hPrp residues 379-424 + spliceosomal protein P14 - NMR<BR />
+
-
[[2do5]] – hPrp subunit 2 – NMR<BR />
+
-
[[1x5t]] - hPrp subunit 4 – NMR<BR />
+
-
[[1x5u]] - hPrp subunit 4 RNA-binding domain – NMR<BR />
+
-
 
+
-
===Pre-mRNA-splicing factor 8===
+
-
 
+
-
[[3sbt]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 – yeast<BR />
+
-
[[3e66]] – yPrp β-finger domain<BR />
+
-
[[3e9o]], [[3e9p]] - yPrp RNA-binding domain<BR />
+
-
[[2p87]], [[2og4]], [[3sbg]] - yPrp C terminal<BR />
+
-
[[3lru]] – hPrp residues 1831-1990<BR />
+
-
[[3e9l]] – hPrp endonuclease domain<BR />
+
-
[[3enb]] - hPrp core domain IV<BR />
+
-
[[2p8r]] – Prp C terminal (mutant) – ''Caenorhabditis elegans''
+
-
 
+
-
===Pre-mRNA-splicing factor 9===
+
-
 
+
-
[[4dgw]] – yPrp + Prp 21 + Prp 11
+
-
 
+
-
===Pre-mRNA-splicing factor 11===
+
-
 
+
-
[[4dgw]] – yPrp + Prp 21 + Prp 9
+
-
 
+
-
===Pre-mRNA-splicing factor 18===
+
-
 
+
-
[[2dk4]] – hPrp splicing factor motif domain - NMR<BR />
+
-
 
+
-
===Pre-mRNA-splicing factor 19===
+
-
 
+
-
[[3lrv]] – yPrp WD40 domain<BR />
+
-
[[2bay]] – yPrp U-box<BR />
+
-
[[1n87]] - yPrp U-box - NMR<BR />
+
-
 
+
-
===Pre-mRNA-splicing factor 20===
+
-
 
+
-
[[2i2y]] – Prp RNA-binding domain + RNA – ''Streptococcus'' – NMR<BR />
+
-
[[2i38]] - hPrp RNA-binding domain (mutant) – NMR<BR />
+
-
 
+
-
===Pre-mRNA-splicing factor 21===
+
-
 
+
-
[[4dgw]] – yPrp + Prp 9 + Prp 11
+
-
 
+
-
===snRNA-associated-splicing factor 24===
+
-
 
+
-
[[2l9w]] – yPrp C terminal – NMR<BR />
+
-
[[2kh9]] – yPrp residues 115-197 + RNA – NMR<BR />
+
-
[[2go9]] – yPrp RNA-binding domains – NMR<BR />
+
-
[[2ghp]] - yPrp RNA-binding domains
+
-
 
+
-
===Pre-mRNA-splicing factor 31===
+
-
 
+
-
[[2ozb]] – hPrp + RNA + tri-snRNP <br />
+
-
[[3siu]], [[3siv]] – hPrp + NHP2-like protein + snRNP <br />
+
-
 
+
-
===Pre-mRNA-splicing factor 40===
+
-
 
+
-
[[1o6w]] – yPrp WW domain – yeast - NMR<br />
+
-
[[2b7e]] – yPrp FF1 domain - NMR<br />
+
-
[[2kfd]] – yPrp FF4 domain - NMR<br />
+
-
 
+
-
===Pre-mRNA-splicing factor 43P===
+
-
 
+
-
[[2xau]], [[3kx2]] – yPrp + ADP
+
-
 
+
-
===Pre-mRNA-splicing factor RBM22===
+
-
 
+
-
[[2ytc]] – yPrp RNA-binding domain - NMR<BR />
+
-
 
+
-
 
+
-
===Pre-mRNA-splicing factor RDS3===
+
-
 
+
-
[[2k0a]] – yPrp - NMR<BR />
+
-
 
+
-
===Pre-mRNA-splicing factor URN1===
+
-
 
+
-
[[2juc]] – yPrp FF domain - NMR<BR />
+
-
 
+
-
 
+
 +
== References ==
 +
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry 3zef)

Drag the structure with the mouse to rotate

References

  1. ↑ Misteli T, Spector DL. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Mol Cell. 1999 Jun;3(6):697-705. PMID:10394358
  2. ↑ McKie AB, McHale JC, Keen TJ, Tarttelin EE, Goliath R, van Lith-Verhoeven JJ, Greenberg J, Ramesar RS, Hoyng CB, Cremers FP, Mackey DA, Bhattacharya SS, Bird AC, Markham AF, Inglehearn CF. Mutations in the pre-mRNA splicing factor gene PRPC8 in autosomal dominant retinitis pigmentosa (RP13). Hum Mol Genet. 2001 Jul 15;10(15):1555-62. PMID:11468273

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

Retrieved from "http://52.214.119.220/wiki/index.php/Pre-mRNA-splicing_factor"
Personal tools