Pre-mRNA-splicing factor

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{{STRUCTURE_3e9l| PDB=3e9l | SIZE=400| SCENE= |right|CAPTION=Human pre-mRNA-splicing factor 8 endonuclease domain complex with Na+ (purple) and Cl- (green) ions, [[3e9l]] }}
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<StructureSection load='' size='350' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'>
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__TOC__
== Function ==
== Function ==
'''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing<ref>PMID:10394358</ref>.
'''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing<ref>PMID:10394358</ref>.
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*'''Prp18''' and '''Bud31''' are essential for the catalytic step in pre-mRNA splicing process.<br />
*'''Prp18''' and '''Bud31''' are essential for the catalytic step in pre-mRNA splicing process.<br />
*'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs. For details see [[Prp24]]<br />
*'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs. For details see [[Prp24]]<br />
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*[[Prp19]]<br />
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*For '''Prp19''' see [[Prp19]]<br />
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*[[Prp40]].<br />
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*For '''Prp20''' see [[Srp20-Human Alternative Splicing Factor]]<br />
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*'''Prp31''' functions in forming the U4/U5-U6 tri-snRNP.<br />
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*For '''Prp40''' see [[Prp40]].<br />
*'''Prpcwc2''' is a yeast splicing factor which plays a role in the generation of a functional splicing center '''Prp RBM22''' is the human homolog of Prpcwc2.<br />
*'''Prpcwc2''' is a yeast splicing factor which plays a role in the generation of a functional splicing center '''Prp RBM22''' is the human homolog of Prpcwc2.<br />
*'''Prpcwc26''' or '''Bud13''' belongs to the pre-mRNA retention and splicing complex RES.<br />
*'''Prpcwc26''' or '''Bud13''' belongs to the pre-mRNA retention and splicing complex RES.<br />
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== 3D Structures of pre-mRNA splicing factors ==
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== Disease ==
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Mutations in Prp8 and Prp31 cause retinitis pigmentosa<ref>PMID:11468273</ref>.
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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== Structural highlights ==
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{{#tree:id=OrganizedByTopic|openlevels=0|
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<scene name='46/462200/Cv/3'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.
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*Pre-mRNA-splicing factor 2
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== 3D Structures of pre-mRNA splicing factors ==
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[[Pre-mRNA splicing factors 3D structures]]
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**[[3beg]] – hPrp P33 subunit + Ser/Thr-protein kinase – human<BR />
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**[[2o3d]] – hPrp P33 subunit
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*Pre-mRNA-splicing factor 3
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**[[1x4q]] – hPrp PWI domain - NMR<BR />
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*Pre-mRNA-splicing factor 3B
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**[[3lqv]], [[2f9d]], [[2f9j]] – hPrp residues 377-415 + pre-mRNA branch site protein 14<BR />
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**[[2fho]] - hPrp residues 379-424 + spliceosomal protein P14 - NMR<BR />
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**[[2do5]] – hPrp subunit 2 – NMR<BR />
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**[[1x5t]] - hPrp subunit 4 – NMR<BR />
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**[[1x5u]] - hPrp subunit 4 RNA-binding domain – NMR<BR />
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*Pre-mRNA-splicing factor 8
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**[[3sbt]], [[4ilh]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 – yeast<BR />
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**[[4jk7]], [[4jk9]] - yPrp RNAseH like domain <br />
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**[[4ilj]], [[4jk8]], [[4jka]], [[4jkb]], [[4jkc]], [[4jkd]], [[4jke]], [[4jkf]], [[4jkg]], [[4jkh]] - yPrp RNAseH like domain (mutant)<br />
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**[[3e66]] – yPrp β-finger domain<BR />
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**[[3e9o]], [[3e9p]] - yPrp RNA-binding domain<BR />
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**[[2p87]], [[2og4]], [[3sbg]] - yPrp C terminal<BR />
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**[[3zef]], [[4i43]] – yPrp + A1 cistron-splicing factor AAR2<br />
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**[[4bgd]] – yPrp JAB1/MPN domain + pre-mRNA-splicing helicase BRR2<br />
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**[[4kit]] – yPrp JAB1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />
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**[[4ilg]] – yPrp JAB1/MPN+RNAseH domains + A1 cistron-splicing factor AAR2 <br />
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**[[4bgd]] – yPrp JAB1/MPN+RNAseH domains + A1 cistron-splicing factor AAR2 + pre-mRNA-splicing helicase BRR2<br />
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**[[3lru]] – hPrp residues 1831-1990<BR />
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**[[3e9l]] – hPrp endonuclease domain<BR />
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**[[3enb]] - hPrp core domain IV<BR />
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**[[2p8r]] – Prp C terminal (mutant) – ''Caenorhabditis elegans''
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*Pre-mRNA-splicing factor 9
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**[[4dgw]] – yPrp + Prp 21 + Prp 11
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*Pre-mRNA-splicing factor 11
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**[[4dgw]] – yPrp + Prp 21 + Prp 9
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*Pre-mRNA-splicing factor 18
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**[[2dk4]] – hPrp splicing factor motif domain - NMR<BR />
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*Pre-mRNA-splicing factor 19
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**[[3lrv]] – yPrp WD40 domain<BR />
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**[[2bay]] – yPrp U-box<BR />
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**[[1n87]] - yPrp U-box - NMR<BR />
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*Pre-mRNA-splicing factor 20
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**[[2i2y]] – Prp RNA-binding domain + RNA – ''Streptococcus'' – NMR<BR />
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**[[2i38]] - hPrp RNA-binding domain (mutant) – NMR<BR />
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*Pre-mRNA-splicing factor 21
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**[[4dgw]] – yPrp + Prp 9 + Prp 11
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*snRNA-associated-splicing factor 24
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**[[2l9w]] – yPrp C terminal – NMR<BR />
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**[[2kh9]] – yPrp residues 115-197 + RNA – NMR<BR />
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**[[2go9]] – yPrp RNA-binding domains – NMR<BR />
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**[[2ghp]] - yPrp RNA-binding domains
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*Pre-mRNA-splicing factor 31
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**[[2ozb]] – hPrp + RNA + tri-snRNP <br />
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**[[3siu]], [[3siv]] – hPrp + NHP2-like protein + snRNP <br />
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*Pre-mRNA-splicing factor 40
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**[[1o6w]] – yPrp WW domain – yeast - NMR<br />
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**[[2b7e]] – yPrp FF1 domain - NMR<br />
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**[[2kfd]] – yPrp FF4 domain - NMR<br />
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*Pre-mRNA-splicing factor 43P
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**[[2xau]], [[3kx2]] – yPrp + ADP
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*Pre-mRNA-splicing factor cwc2
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**[[3tp2]], [[3u1l]], [[3u1m]] – yPrp <br />
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*Pre-mRNA-splicing factor cwc26
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**[[2mkc]] – yPrp + U2 SNRNP component IST3 + Pre-mRNA leakage protein<br />
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**[[4uqt]] – yPrp + U2 SNRNP component IST3 <br />
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*Pre-mRNA-splicing factor bud31
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**[[2my1]] – yPrp - NMR<br />
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*Pre-mRNA-splicing factor sad1
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**[[4msx]] – yPrp <br />
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*Pre-mRNA-splicing factor RBM22
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**[[2ytc]] – yPrp RNA-binding domain - NMR<BR />
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*Pre-mRNA-splicing factor RDS3
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**[[2k0a]] – yPrp - NMR<BR />
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*Pre-mRNA-splicing factor URN1
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**[[2juc]] – yPrp FF domain - NMR<BR />
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</StructureSection>
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}}
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== References ==
== References ==

Current revision

Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry 3zef)

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References

  1. ↑ Misteli T, Spector DL. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Mol Cell. 1999 Jun;3(6):697-705. PMID:10394358
  2. ↑ McKie AB, McHale JC, Keen TJ, Tarttelin EE, Goliath R, van Lith-Verhoeven JJ, Greenberg J, Ramesar RS, Hoyng CB, Cremers FP, Mackey DA, Bhattacharya SS, Bird AC, Markham AF, Inglehearn CF. Mutations in the pre-mRNA splicing factor gene PRPC8 in autosomal dominant retinitis pigmentosa (RP13). Hum Mol Genet. 2001 Jul 15;10(15):1555-62. PMID:11468273

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