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6mks

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Revision as of 10:59, 18 December 2019

Cryo-EM structure of NLRC4-CARD filament

Structural highlights

6mks is a 31 chain structure with sequence from Aeqvi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	EGFP (AEQVI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[NLRC4_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.

Function

[NLRC4_HUMAN] Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).[UniProtKB:Q3UP24]^[1]

Publication Abstract from PubMed

Inflammasomes are supramolecular signaling platforms integral to innate immune defense against invading pathogens. The Nod Like Receptor (NLR) family apoptosis inhibitory protein (NAIP)*NLR family caspase-recruiting domain (CARD) domain-containing 4 (NLRC4) inflammasome recognizes intracellular bacteria and induces the polymerization of the caspase-1 protease, which in turn executes maturation of interleukin-1beta (IL-1beta) and pyroptosis. Several high-resolution structures of the fully assembled NAIP*NLRC4 complex are available, but these structures do not resolve the architecture of the CARD filament in atomic detail. Here, we present the cryo-EM structure of the filament assembled by the CARD of human NLRC4 (NLRC4(CARD)) at 3.4 A resolution. The structure revealed that the helical architecture of the NLRC4(CARD) filament is essentially identical to that of the downstream filament assembled by the CARD of caspase-1 (casp1(CARD)), but deviates from the split washer-like assembly of the NAIP*NLRC4 oligomer. Our results suggest that architectural complementarity is a major driver for the recognition between up- and downstream CARD assemblies in inflammasomes. Furthermore, a Monte Carlo simulation of the NLRC4(CARD) filament assembly rationalizes why an (un)decameric NLRC4 oligomer is optimal for assembling the helical base of the NLRC4(CARD) filament. Together, our results explain how symmetric and asymmetric supramolecular assemblies enable high-fidelity signaling in inflammasomes.

Cryo-EM structure of the NLRC4(CARD) filament provides insights into how symmetric and asymmetric supramolecular structures drive inflammasome assembly.,Matyszewski M, Zheng W, Lueck J, Antiochos B, Egelman EH, Sohn J J Biol Chem. 2018 Nov 1. pii: RA118.006050. doi: 10.1074/jbc.RA118.006050. PMID:30385506^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Damiano JS, Oliveira V, Welsh K, Reed JC. Heterotypic interactions among NACHT domains: implications for regulation of innate immune responses. Biochem J. 2004 Jul 1;381(Pt 1):213-9. doi: 10.1042/BJ20031506. PMID:15107016 doi:http://dx.doi.org/10.1042/BJ20031506
↑ Matyszewski M, Zheng W, Lueck J, Antiochos B, Egelman EH, Sohn J. Cryo-EM structure of the NLRC4(CARD) filament provides insights into how symmetric and asymmetric supramolecular structures drive inflammasome assembly. J Biol Chem. 2018 Nov 1. pii: RA118.006050. doi: 10.1074/jbc.RA118.006050. PMID:30385506 doi:http://dx.doi.org/10.1074/jbc.RA118.006050

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6mks"

@@ Line 1: / Line 1: @@
 ==Cryo-EM structure of NLRC4-CARD filament==
-<StructureSection load='6mks' size='340' side='right' caption='[[6mks]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+<StructureSection load='6mks' size='340' side='right'caption='[[6mks]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6mks]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MKS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6mks]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MKS FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EGFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EGFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mks OCA], [http://pdbe.org/6mks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mks RCSB], [http://www.ebi.ac.uk/pdbsum/6mks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mks ProSAT]</span></td></tr>
 </table>
@@ Line 20: / Line 20: @@
 </div>
 <div class="pdbe-citations 6mks" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Aeqvi]]
+[[Category: Large Structures]]
 [[Category: Egelman, E H]]
 [[Category: Matyszewski, M]]

6mks

From Proteopedia

Revision as of 10:59, 18 December 2019

Cryo-EM structure of NLRC4-CARD filament

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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