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6p1c
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Transcription antitermination factor Q21, SeMet-derivative== | |
| + | <StructureSection load='6p1c' size='340' side='right'caption='[[6p1c]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6p1c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_21 Bacteriophage 21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P1C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P1C FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p1c OCA], [http://pdbe.org/6p1c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p1c RCSB], [http://www.ebi.ac.uk/pdbsum/6p1c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p1c ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lambdoid bacteriophage Q protein mediates the switch from middle to late bacteriophage gene expression by enabling RNA polymerase (RNAP) to read through transcription terminators preceding bacteriophage late genes. Q loads onto RNAP engaged in promoter-proximal pausing at a Q binding element (QBE) and adjacent sigma-dependent pause element (SDPE) to yield a Q-loading complex, and Q subsequently translocates with RNAP as a pausing-deficient, termination-deficient Q-loaded complex. Here, we report high-resolution structures of 4 states on the pathway of antitermination by Q from bacteriophage 21 (Q21): Q21, the Q21-QBE complex, the Q21-loading complex, and the Q21-loaded complex. The results show that Q21 forms a torus, a "nozzle," that narrows and extends the RNAP RNA-exit channel, extruding topologically linked single-stranded RNA and preventing the formation of pause and terminator hairpins. | ||
| - | + | Structural basis of Q-dependent antitermination.,Yin Z, Kaelber JT, Ebright RH Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18384-18390. doi:, 10.1073/pnas.1909801116. Epub 2019 Aug 27. PMID:31455742<ref>PMID:31455742</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Ebright, R | + | <div class="pdbe-citations 6p1c" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacteriophage 21]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ebright, R H]] | ||
[[Category: Yin, Z]] | [[Category: Yin, Z]] | ||
| + | [[Category: Dna binding]] | ||
| + | [[Category: Gene regulation]] | ||
| + | [[Category: Q antitermination factor]] | ||
| + | [[Category: Q-dependent antitermination]] | ||
| + | [[Category: Rna polymerase]] | ||
| + | [[Category: Transcription]] | ||
Current revision
Transcription antitermination factor Q21, SeMet-derivative
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