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6k96
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Ari2== | |
| + | <StructureSection load='6k96' size='340' side='right'caption='[[6k96]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6k96]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K96 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k96 OCA], [http://pdbe.org/6k96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k96 RCSB], [http://www.ebi.ac.uk/pdbsum/6k96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k96 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The myo-inositol-1-phosphate synthase (MIPS) ortholog Ari2, which is encoded in the aristeromycin biosynthetic gene cluster, catalyzes the formation of five-membered cyclitol phosphate using d-fructose 6-phosphate (F6P) as a substrate. To understand the stereochemistry during the Ari2 reaction in vivo, we carried out feeding experiments with (6S)-d-[6-(2)H1]- and (6R)-d-[6-(2)H1]glucose in the aristeromycin-producing strain Streptomyces citricolor. We observed retention of the (2)H atom of (6S)-d-[6-(2)H1]glucose and no incorporation of the (2)H atom from (6R)-d-[6-(2)H1]glucose in aristeromycin. This indicates that Ari2 abstracts the pro-R proton at C6 of F6P after oxidation of C5-OH by nicotinamide adenine dinucleotide (NAD(+)) to generate the enolate intermediate, which then attacks the C2 ketone to form the C-C bond via aldol-type condensation. The reaction of Ari2 with (6S)-d-[6-(2)H1]- and (6R)-d-[6-(2)H1]F6P in vitro exhibited identical stereochemistry compared with that observed during the feeding experiments. Furthermore, analysis of the crystal structure of Ari2, including NAD(+) as a ligand, revealed the active site of Ari2 to be similar to that of MIPS of Mycobacterium tuberculosis, supporting the similarity of the reaction mechanisms of Ari2 and MIPS. | ||
| - | + | Stereochemistry in the Reaction of the myo-Inositol Phosphate Synthase Ortholog Ari2 during Aristeromycin Biosynthesis.,Kudo F, Tsunoda T, Yamaguchi K, Miyanaga A, Eguchi T Biochemistry. 2019 Dec 24;58(51):5112-5116. doi: 10.1021/acs.biochem.9b00981., Epub 2019 Dec 13. PMID:31825604<ref>PMID:31825604</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6k96" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Eguchi, T]] | ||
| + | [[Category: Kudo, F]] | ||
| + | [[Category: Miyanaga, A]] | ||
| + | [[Category: Tsunoda, T]] | ||
| + | [[Category: Nad binding]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Rossmann fold]] | ||
Revision as of 09:04, 25 December 2019
Crystal structure of Ari2
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