This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5v8d
From Proteopedia
(Difference between revisions)
m (Protected "5v8d" [edit=sysop:move=sysop]) |
|||
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Bacillus cereus PatB1 with sulfonyl adduct== | |
| + | <StructureSection load='5v8d' size='340' side='right'caption='[[5v8d]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5v8d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacc1 Bacc1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V8D FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OSE:O-SULFO-L-SERINE'>OSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5v8e|5v8e]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BCE_0974 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=222523 BACC1])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8d OCA], [http://pdbe.org/5v8d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v8d RCSB], [http://www.ebi.ac.uk/pdbsum/5v8d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8d ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1. | ||
| - | + | PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides.,Sychantha D, Little DJ, Chapman RN, Boons GJ, Robinson H, Howell PL, Clarke AJ Nat Chem Biol. 2017 Oct 30. pii: nchembio.2509. doi: 10.1038/nchembio.2509. PMID:29083419<ref>PMID:29083419</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5v8d" style="background-color:#fffaf0;"></div> |
| - | [[Category: Boons, G | + | == References == |
| - | [[Category: Clarke, A | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacc1]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Boons, G J]] | ||
| + | [[Category: Chapman, R N]] | ||
| + | [[Category: Clarke, A J]] | ||
| + | [[Category: Howell, P L]] | ||
| + | [[Category: Little, D J]] | ||
[[Category: Robinson, H]] | [[Category: Robinson, H]] | ||
[[Category: Sychantha, D]] | [[Category: Sychantha, D]] | ||
| - | [[Category: | + | [[Category: Acetyltransferase]] |
| - | [[Category: | + | [[Category: Cell wall]] |
| + | [[Category: Sgnh hydrolase-like]] | ||
| + | [[Category: Transferase]] | ||
Current revision
Structure of Bacillus cereus PatB1 with sulfonyl adduct
| |||||||||||
