1acc
From Proteopedia
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'''ANTHRAX PROTECTIVE ANTIGEN''' | '''ANTHRAX PROTECTIVE ANTIGEN''' | ||
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[[Category: Liddington, R C.]] | [[Category: Liddington, R C.]] | ||
[[Category: Petosa, C.]] | [[Category: Petosa, C.]] | ||
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| - | [[Category: | + | [[Category: Toxin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:05:56 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:05, 2 May 2008
ANTHRAX PROTECTIVE ANTIGEN
Overview
Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax. After proteolytic activation on the host cell surface, PA forms a membrane-inserting heptamer that translocates the toxic enzymes, oedema factor and lethal factor, into the cytosol. PA, which has a relative molecular mass of 83,000 (M(r) 83K), can also translocate heterologous proteins, and is being evaluated for use as a general protein delivery system. Here we report the crystal structure of monomeric PA at 2.1 A resolution and the water-soluble heptamer at 4.5 A resolution. The monomer is organized mainly into antiparallel beta-sheets and has four domains: an amino-terminal domain (domain 1) containing two calcium ions and the cleavage site for activating proteases; a heptamerization domain (domain 2) containing a large flexible loop implicated in membrane insertion; a small domain of unknown function (domain 3); and a carboxy-terminal receptor-binding domain (domain 4). Removal of a 20K amino-terminal fragment from domain 1 allows the assembly of the heptamer, a ring-shaped structure with a negatively charged lumen, and exposes a large hydrophobic surface for binding the toxic enzymes. We propose a model of pH-dependent membrane insertion involving the formation of a porin-like, membrane-spanning beta-barrel.
About this Structure
1ACC is a Single protein structure of sequence from Bacillus anthracis. The following page contains interesting information on the relation of 1ACC with [Anthrax Toxin]. Full crystallographic information is available from OCA.
Reference
Crystal structure of the anthrax toxin protective antigen., Petosa C, Collier RJ, Klimpel KR, Leppla SH, Liddington RC, Nature. 1997 Feb 27;385(6619):833-8. PMID:9039918 Page seeded by OCA on Fri May 2 10:05:56 2008
