1af4

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[[Image:1af4.gif|left|200px]]
[[Image:1af4.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1af4 |SIZE=350|CAPTION= <scene name='initialview01'>1af4</scene>, resolution 2.6&Aring;
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The line below this paragraph, containing "STRUCTURE_1af4", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1af4| PDB=1af4 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1af4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af4 OCA], [http://www.ebi.ac.uk/pdbsum/1af4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1af4 RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF SUBTILISIN CARLSBERG IN ANHYDROUS DIOXANE'''
'''CRYSTAL STRUCTURE OF SUBTILISIN CARLSBERG IN ANHYDROUS DIOXANE'''
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[[Category: Schmitke, J L.]]
[[Category: Schmitke, J L.]]
[[Category: Stern, L J.]]
[[Category: Stern, L J.]]
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[[Category: organic solvent]]
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[[Category: Organic solvent]]
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[[Category: serine protease]]
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[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:11:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:38:52 2008''
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Revision as of 07:11, 2 May 2008

Image:1af4.gif

Template:STRUCTURE 1af4

CRYSTAL STRUCTURE OF SUBTILISIN CARLSBERG IN ANHYDROUS DIOXANE


Overview

The x-ray crystal structure of the serine protease subtilisin Carlsberg in anhydrous dioxane has been determined to 2.6-A resolution. The enzyme structure is found to be nearly indistinguishable from the structures previously determined in water and acetonitrile. Small changes in the side-chain conformations between the dioxane and water structures are of the same magnitude as those observed between two structures in different aqueous systems. Seven enzyme-bound dioxane molecules have been detected, each potentially forming at least one hydrogen bond with a subtilisin hydrogen-bond donor or bound water. Two of the bound dioxane molecules are in the active-site region, one in the P2 and another bridging the P1' and P3' pockets. The other five dioxane molecules are located on the surface of subtilisin at interprotein crystal contacts. The locations of the bound solvent in the dioxane structure are distinct from those in the structures in acetonitrile and in water.

About this Structure

1AF4 is a Single protein structure of sequence from Bacillus licheniformis. Full crystallographic information is available from OCA.

Reference

The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile., Schmitke JL, Stern LJ, Klibanov AM, Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4250-5. PMID:9113975 Page seeded by OCA on Fri May 2 10:11:08 2008

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