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5hyw

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Revision as of 07:16, 19 February 2020

The crystal structure of the D3-ASK1 complex

Structural highlights

5hyw is a 4 chain structure with sequence from Arath and Japanese rice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5hzg
Gene:	D3, Os06g0154200, LOC_Os06g06050, OSJNBa0085L11.6-1 (Japanese rice), SKP1A, ASK1, SKP1, UIP1, At1g75950, T4O12.17 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MAX2_ORYSJ] Controls tillering by suppressing axillary bud activity. Tiller is a specialized grain-bearing branch that is formed on the unelongated basal internode and grows independently of the mother stem (culm) by means of its own adventitious roots. Is necessary for responses to strigolactones and the establishment of arbuscular mycorrhiza symbiosis in rice. May be involved in the ubiquitin-mediated degradation of specific proteins that activate axillary growth. D3 is crucial for establishing arbuscular mycorrhiza (AM) symbiosis in rice, whereas D14 and D14-LIKE are not. Our results suggest distinct roles for these SL-related components in AM symbiosis. [SKP1A_ARATH] Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends of the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

Publication Abstract from PubMed

Classical hormone receptors reversibly and non-covalently bind active hormone molecules, which are generated by biosynthetic enzymes, to trigger signal transduction. The alpha/beta hydrolase DWARF14 (D14), which hydrolyses the plant branching hormone strigolactone and interacts with the F-box protein D3/MAX2, is probably involved in strigolactone detection. However, the active form of strigolactone has yet to be identified and it is unclear which protein directly binds the active form of strigolactone, and in which manner, to act as the genuine strigolactone receptor. Here we report the crystal structure of the strigolactone-induced AtD14-D3-ASK1 complex, reveal that Arabidopsis thaliana (At)D14 undergoes an open-to-closed state transition to trigger strigolactone signalling, and demonstrate that strigolactone is hydrolysed into a covalently linked intermediate molecule (CLIM) to initiate a conformational change of AtD14 to facilitate interaction with D3. Notably, analyses of a highly branched Arabidopsis mutant d14-5 show that the AtD14(G158E) mutant maintains enzyme activity to hydrolyse strigolactone, but fails to efficiently interact with D3/MAX2 and loses the ability to act as a receptor that triggers strigolactone signalling in planta. These findings uncover a mechanism underlying the allosteric activation of AtD14 by strigolactone hydrolysis into CLIM, and define AtD14 as a non-canonical hormone receptor with dual functions to generate and sense the active form of strigolactone.

DWARF14 is a non-canonical hormone receptor for strigolactone.,Yao R, Ming Z, Yan L, Li S, Wang F, Ma S, Yu C, Yang M, Chen L, Chen L, Li Y, Yan C, Miao D, Sun Z, Yan J, Sun Y, Wang L, Chu J, Fan S, He W, Deng H, Nan F, Li J, Rao Z, Lou Z, Xie D Nature. 2016 Aug 25;536(7617):469-73. PMID:27479325^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhao D, Yang M, Solava J, Ma H. The ASK1 gene regulates development and interacts with the UFO gene to control floral organ identity in Arabidopsis. Dev Genet. 1999 Sep;25(3):209-23. PMID:10528262 doi:<209::AID-DVG4>3.0.CO;2-O 10.1002/(SICI)1520-6408(1999)25:3<209::AID-DVG4>3.0.CO;2-O
↑ Gray WM, del Pozo JC, Walker L, Hobbie L, Risseeuw E, Banks T, Crosby WL, Yang M, Ma H, Estelle M. Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana. Genes Dev. 1999 Jul 1;13(13):1678-91. PMID:10398681
↑ Yang M, Hu Y, Lodhi M, McCombie WR, Ma H. The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11416-21. PMID:10500191
↑ Zhao D, Yu Q, Chen M, Ma H. The ASK1 gene regulates B function gene expression in cooperation with UFO and LEAFY in Arabidopsis. Development. 2001 Jul;128(14):2735-46. PMID:11526079
↑ Zhao D, Ni W, Feng B, Han T, Petrasek MG, Ma H. Members of the Arabidopsis-SKP1-like gene family exhibit a variety of expression patterns and may play diverse roles in Arabidopsis. Plant Physiol. 2003 Sep;133(1):203-17. PMID:12970487
↑ Liu F, Ni W, Griffith ME, Huang Z, Chang C, Peng W, Ma H, Xie D. The ASK1 and ASK2 genes are essential for Arabidopsis early development. Plant Cell. 2004 Jan;16(1):5-20. Epub 2003 Dec 19. PMID:14688296 doi:10.1105/tpc.017772
↑ Yao R, Ming Z, Yan L, Li S, Wang F, Ma S, Yu C, Yang M, Chen L, Chen L, Li Y, Yan C, Miao D, Sun Z, Yan J, Sun Y, Wang L, Chu J, Fan S, He W, Deng H, Nan F, Li J, Rao Z, Lou Z, Xie D. DWARF14 is a non-canonical hormone receptor for strigolactone. Nature. 2016 Aug 25;536(7617):469-73. PMID:27479325 doi:http://dx.doi.org/10.1038/nature19073

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5hyw"

@@ Line 1: / Line 1: @@
 ==The crystal structure of the D3-ASK1 complex==
-<StructureSection load='5hyw' size='340' side='right' caption='[[5hyw]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
+<StructureSection load='5hyw' size='340' side='right'caption='[[5hyw]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5hyw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HYW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5hyw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath] and [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HYW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HYW FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hzg|5hzg]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">D3, Os06g0154200, LOC_Os06g06050, OSJNBa0085L11.6-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice]), SKP1A, ASK1, SKP1, UIP1, At1g75950, T4O12.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hyw OCA], [http://pdbe.org/5hyw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hyw RCSB], [http://www.ebi.ac.uk/pdbsum/5hyw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hyw ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Arath]]
+[[Category: Japanese rice]]
+[[Category: Large Structures]]
 [[Category: Lou, Z Y]]
 [[Category: Ming, Z H]]

5hyw

From Proteopedia

Revision as of 07:16, 19 February 2020

The crystal structure of the D3-ASK1 complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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