1ako

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[[Image:1ako.gif|left|200px]]
[[Image:1ako.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ako |SIZE=350|CAPTION= <scene name='initialview01'>1ako</scene>, resolution 1.7&Aring;
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The line below this paragraph, containing "STRUCTURE_1ako", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=MG1:Mg+Binding+Site'>MG1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= XTH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1ako| PDB=1ako | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ako FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ako OCA], [http://www.ebi.ac.uk/pdbsum/1ako PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ako RCSB]</span>
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}}
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'''EXONUCLEASE III FROM ESCHERICHIA COLI'''
'''EXONUCLEASE III FROM ESCHERICHIA COLI'''
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[[Category: Tainer, J A.]]
[[Category: Tainer, J A.]]
[[Category: Thayer, M M.]]
[[Category: Thayer, M M.]]
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[[Category: ap-endonuclease]]
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[[Category: Ap-endonuclease]]
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[[Category: dna repair]]
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[[Category: Dna repair]]
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[[Category: exonuclease]]
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[[Category: Exonuclease]]
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[[Category: nuclease]]
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[[Category: Nuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:23:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:42:16 2008''
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Revision as of 07:23, 2 May 2008

Image:1ako.gif

Template:STRUCTURE 1ako

EXONUCLEASE III FROM ESCHERICHIA COLI


Overview

The repair of DNA requires the removal of abasic sites, which are constantly generated in vivo both spontaneously and by enzymatic removal of uracil, and of bases damaged by active oxygen species, alkylating agents and ionizing radiation. The major apurinic/apyrimidinic (AP) DNA-repair endonuclease in Escherichia coli is the multifunctional enzyme exonuclease III, which also exhibits 3'-repair diesterase, 3'-->5' exonuclease, 3'-phosphomonoesterase and ribonuclease activities. We report here the 1.7 A resolution crystal structure of exonuclease III which reveals a 2-fold symmetric, four-layered alpha beta fold with similarities to both deoxyribonuclease I and RNase H. In the ternary complex determined at 2.6 A resolution, Mn2+ and dCMP bind to exonuclease III at one end of the alpha beta-sandwich, in a region dominated by positive electrostatic potential. Residues conserved among AP endonucleases from bacteria to man cluster within this active site and appear to participate in phosphate-bond cleavage at AP sites through a nucleophilic attack facilitated by a single bound metal ion.

About this Structure

1AKO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure and function of the multifunctional DNA-repair enzyme exonuclease III., Mol CD, Kuo CF, Thayer MM, Cunningham RP, Tainer JA, Nature. 1995 Mar 23;374(6520):381-6. PMID:7885481 Page seeded by OCA on Fri May 2 10:23:31 2008

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