This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

6i52

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 22:00, 6 March 2020

Yeast RPA bound to ssDNA

6i52, resolution 4.70Å

Structural highlights

6i52 is a 4 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	RFA3, YJL173C, J0506 (Baker's yeast), RFA2, BUF1, YNL312W, N0368 (Baker's yeast), RFA1, BUF2, RPA1, YAR007C, FUN3 (Baker's yeast)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RFA3_YEAST] As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism (By similarity). Stimulates the activity of a cognate strand exchange protein (SEP1). [RFA1_YEAST] Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication. [RFA2_YEAST] Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.

Publication Abstract from PubMed

Replication Protein A (RPA), the major eukaryotic single stranded DNA-binding protein, binds to exposed ssDNA to protect it from nucleases, participates in a myriad of nucleic acid transactions and coordinates the recruitment of other important players. RPA is a heterotrimer and coats long stretches of single-stranded DNA (ssDNA). The precise molecular architecture of the RPA subunits and its DNA binding domains (DBDs) during assembly is poorly understood. Using cryo electron microscopy we obtained a 3D reconstruction of the RPA trimerisation core bound with ssDNA ( approximately 55 kDa) at approximately 4.7 A resolution and a dimeric RPA assembly on ssDNA. FRET-based solution studies reveal dynamic rearrangements of DBDs during coordinated RPA binding and this activity is regulated by phosphorylation at S178 in RPA70. We present a structural model on how dynamic DBDs promote the cooperative assembly of multiple RPAs on long ssDNA.

A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA.,Yates LA, Aramayo RJ, Pokhrel N, Caldwell CC, Kaplan JA, Perera RL, Spies M, Antony E, Zhang X Nat Commun. 2018 Dec 21;9(1):5447. doi: 10.1038/s41467-018-07883-7. PMID:30575763^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yates LA, Aramayo RJ, Pokhrel N, Caldwell CC, Kaplan JA, Perera RL, Spies M, Antony E, Zhang X. A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA. Nat Commun. 2018 Dec 21;9(1):5447. doi: 10.1038/s41467-018-07883-7. PMID:30575763 doi:http://dx.doi.org/10.1038/s41467-018-07883-7

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6i52"

@@ Line 1: / Line 1: @@
 ==Yeast RPA bound to ssDNA==
-<StructureSection load='6i52' size='340' side='right' caption='[[6i52]], [[Resolution|resolution]] 4.70&Aring;' scene=''>
+<SX load='6i52' size='340' side='right' viewer='molstar' caption='[[6i52]], [[Resolution|resolution]] 4.70&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[6i52]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I52 FirstGlance]. <br>
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 6i52" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
 == References ==
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Aramayo, R J]]
 [[Category: Yates, L A]]

6i52

From Proteopedia

Revision as of 22:00, 6 March 2020

Yeast RPA bound to ssDNA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox