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6kol

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Revision as of 10:15, 18 March 2020

Crystal structure of auracyanin from photosynthetic bacterium Roseiflexus castenholzii

Structural highlights

6kol is a 1 chain structure with sequence from Roscs. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Auracyanin (Ac) is a blue copper protein that mediates the electron transfer between Alternative Complex III (ACIII) and downstream electron acceptors in both fort chains of filamentous anoxygenic phototrophs. Here, we extracted and purified the air-oxidized RfxAc from the photoheterotrophically grown Roseiflexus castenholzii, and we illustrated the structural basis underlying its electron transferring features. Spectroscopic and enzymatic analyses demonstrated the reduction of air-oxidized RfxAc by the ACIII upon oxidation of menaquinol-4 and menaquinol-7. Crystal structures of the air-oxidized and Na-dithionite-reduced RfxAc at 2.2 and 2.0 A resolutions, respectively, showed that the copper ions are coordinated by His77, His146, Cys141, and Met151 in minor different geometries. The Cu(1)-S(delta) bond length increase of Met151, and the electron density Fourier differences at Cu(1) and His77 demonstrated their essential roles in the dithionite-induced reduction. Structural comparisons further revealed that the RfxAc contains a Chloroflexus aurantiacus Ac-A-like copper binding pocket and a hydrophobic patch surrounding the exposed edge of His146 imidazole, as well as an Ac-B-like Ser- and Thr-rich polar patch located at a different site on the surface. These spectroscopic and structural features allow RfxAc to mediate electron transfers between the ACIII and redox partners different from those of Ac-A and Ac-B. These results provide a structural basis for further investigating the electron transfer and energy transformation mechanism of bacterial photosynthesis, and the diversity and evolution of electron transport chains.

Structural basis underlying the electron transfer features of a blue copper protein auracyanin from the photosynthetic bacterium Roseiflexus castenholzii.,Wang C, Xin Y, Min Z, Qi J, Zhang C, Xu X Photosynth Res. 2020 Jan 13. pii: 10.1007/s11120-020-00709-y. doi:, 10.1007/s11120-020-00709-y. PMID:31933173^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang C, Xin Y, Min Z, Qi J, Zhang C, Xu X. Structural basis underlying the electron transfer features of a blue copper protein auracyanin from the photosynthetic bacterium Roseiflexus castenholzii. Photosynth Res. 2020 Jan 13. pii: 10.1007/s11120-020-00709-y. doi:, 10.1007/s11120-020-00709-y. PMID:31933173 doi:http://dx.doi.org/10.1007/s11120-020-00709-y

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6kol"

@@ Line 3: / Line 3: @@
 <StructureSection load='6kol' size='340' side='right'caption='[[6kol]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6kol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Roseiflexus_castenholzii_(strain_dsm_13941_/_hlo8) Roseiflexus castenholzii (strain dsm 13941 / hlo8)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KOL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6kol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Roscs Roscs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KOL FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kol OCA], [http://pdbe.org/6kol PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kol RCSB], [http://www.ebi.ac.uk/pdbsum/6kol PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kol ProSAT]</span></td></tr>
@@ Line 21: / Line 21: @@
 </StructureSection>
 [[Category: Large Structures]]
+[[Category: Roscs]]
 [[Category: Min, Z Z]]
 [[Category: Wang, C]]

6kol

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Revision as of 10:15, 18 March 2020

Crystal structure of auracyanin from photosynthetic bacterium Roseiflexus castenholzii

Structural highlights

Publication Abstract from PubMed

References

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