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6pcm
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Mycobacterium smegmatis Topoisomerase I with ssDNA bound to both N- and C-terminal domains== | |
| - | + | <StructureSection load='6pcm' size='340' side='right'caption='[[6pcm]], [[Resolution|resolution]] 3.11Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[6pcm]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PCM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PCM FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |
| - | [[Category: | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.6.2.2 5.6.2.2] </span></td></tr> |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pcm OCA], [http://pdbe.org/6pcm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pcm RCSB], [http://www.ebi.ac.uk/pdbsum/6pcm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pcm ProSAT]</span></td></tr> |
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/TOP1_MYCS2 TOP1_MYCS2]] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate (PubMed:9593741) and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).[HAMAP-Rule:MF_00952]<ref>PMID:9593741</ref> Relaxes negatively (but not positively) supercoiled DNA, concatanates and knots circular ssDNA at 52 but not 37 degrees Celsius (PubMed:9593741). Preferentially nicks supercoiled DNA at C(G/T)CTT, cutting between the TT residues, binds ss and dsDNA with the recognition site (PubMed:10734203).<ref>PMID:10734203</ref> <ref>PMID:9593741</ref> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: DNA topoisomerase]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cao, N]] | ||
[[Category: Tan, K]] | [[Category: Tan, K]] | ||
| - | [[Category: | + | [[Category: Tse-Dinh, Y C]] |
| + | [[Category: C-terminal domain]] | ||
| + | [[Category: Isomerase-dna complex]] | ||
| + | [[Category: N-terminal domain]] | ||
| + | [[Category: Ss dna binding]] | ||
| + | [[Category: Topoisomerase i]] | ||
Revision as of 10:12, 27 March 2020
Crystal Structure of Mycobacterium smegmatis Topoisomerase I with ssDNA bound to both N- and C-terminal domains
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