6rv5
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray structure of the levansucrase from Erwinia tasmaniensis in complex with levanbiose== | |
| + | <StructureSection load='6rv5' size='340' side='right'caption='[[6rv5]], [[Resolution|resolution]] 1.58Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6rv5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RV5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rv5 OCA], [http://pdbe.org/6rv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rv5 RCSB], [http://www.ebi.ac.uk/pdbsum/6rv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rv5 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Erwinia tasmaniensis is an epiphytic bacterium related to the plant pathogen Erwinia amylovora, the etiological agent of fire blight. In this study the levansucrase from E. tasmaniensis (EtLsc) has been compared with the homologous enzyme from E. amylovora (EaLsc). We characterized the enzymatic activity and compared the products profile of both enzymes by High Performance Anion Exchange Chromatography coupled with Pulsed Amperometric Detector (HPAEC-PAD). Moreover we determined the crystal structure of EtLsc to understand the structural peculiarity causing the different product profiles of the two homologues. EtLsc exhibits increased efficiency in the production of FOS, resulting in a better catalyst for biotechnological synthesis than EaLsc. Based on our results, we propose that the role of this enzyme in the life cycle of the two bacteria is most likely related to survival, rather than linked to pathogenicity in E. amylovora. | ||
| - | + | Comparison of the Levansucrase from the epiphyte Erwinia tasmaniensis vs its homologue from the phytopathogen Erwinia amylovora.,Polsinelli I, Caliandro R, Salomone-Stagni M, Demitri N, Rejzek M, Field RA, Benini S Int J Biol Macromol. 2019 Apr 15;127:496-501. doi:, 10.1016/j.ijbiomac.2019.01.074. Epub 2019 Jan 17. PMID:30660564<ref>PMID:30660564</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6rv5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Levansucrase]] | ||
| + | [[Category: Benini, S]] | ||
[[Category: Caliandro, R]] | [[Category: Caliandro, R]] | ||
| - | [[Category: Polsinelli, I]] | ||
[[Category: Demitri, N]] | [[Category: Demitri, N]] | ||
| - | [[Category: | + | [[Category: Polsinelli, I]] |
| + | [[Category: Fructans production]] | ||
| + | [[Category: Fructosyltransferase]] | ||
| + | [[Category: Levanbiose]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 09:20, 1 April 2020
X-ray structure of the levansucrase from Erwinia tasmaniensis in complex with levanbiose
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