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5di3

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Revision as of 08:04, 8 April 2020

Crystal structure of Arl13B in complex with Arl3 of Chlamydomonas reinhardtii

Structural highlights

5di3 is a 2 chain structure with sequence from Chlre. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	ARL13, CHLREDRAFT_195529 (CHLRE), ARL3, CHLREDRAFT_128761 (CHLRE)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ARL13_CHLRE] Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B (By similarity). [ARL3_CHLRE] Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Required for targeting proteins to the ciliary membrane by releasing myristoylated protein from unc119 cargo adapters into the cilium.[UniProtKB:P36405]

Publication Abstract from PubMed

Small G-proteins of the ADP-ribosylation-factor-like (Arl) subfamily have been shown to be crucial to ciliogenesis and cilia maintenance. Active Arl3 is involved in targeting and releasing lipidated cargo proteins from their carriers PDE6delta and UNC119a/b to the cilium. However, the Guanine-Nucleotide-Exchange-factor (GEF) which activates Arl3 is unknown. Here we show that the ciliary G-protein Arl13B mutated in Joubert-Syndrome is the GEF for Arl3 and its function is conserved in evolution. The GEF activity of Arl13B is mediated by the G-domain plus an additional C-terminal helix. The switch regions of Arl13B are involved in the interaction with Arl3. Overexpression of Arl13B in mammalian cell lines leads to an increased Arl3*GTP level, whereas Arl13B Joubert-Syndrome patient mutations impair GEF activity and thus Arl3 activation. We anticipate that through Arl13B's exclusive ciliary localization, Arl3 activation is spatially restricted and thereby an Arl3*GTP compartment generated where ciliary cargo is specifically released.

A G-protein activation cascade from Arl13B to Arl3 and implications for ciliary targeting of lipidated proteins.,Gotthardt K, Lokaj M, Koerner C, Falk N, Giessl A, Wittinghofer A Elife. 2015 Nov 9;4. pii: e11859. doi: 10.7554/eLife.11859. PMID:26551564^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gotthardt K, Lokaj M, Koerner C, Falk N, Giessl A, Wittinghofer A. A G-protein activation cascade from Arl13B to Arl3 and implications for ciliary targeting of lipidated proteins. Elife. 2015 Nov 9;4. pii: e11859. doi: 10.7554/eLife.11859. PMID:26551564 doi:http://dx.doi.org/10.7554/eLife.11859

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5di3"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Arl13B in complex with Arl3 of Chlamydomonas reinhardtii==
-<StructureSection load='5di3' size='340' side='right' caption='[[5di3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='5di3' size='340' side='right'caption='[[5di3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5di3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DI3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5di3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DI3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DI3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5di3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5di3 OCA], [http://pdbe.org/5di3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5di3 RCSB], [http://www.ebi.ac.uk/pdbsum/5di3 PDBsum]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARL13, CHLREDRAFT_195529 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE]), ARL3, CHLREDRAFT_128761 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5di3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5di3 OCA], [http://pdbe.org/5di3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5di3 RCSB], [http://www.ebi.ac.uk/pdbsum/5di3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5di3 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Chlre]]
+[[Category: Large Structures]]
 [[Category: Falk, N]]
 [[Category: Giessl, A]]

5di3

From Proteopedia

Revision as of 08:04, 8 April 2020

Crystal structure of Arl13B in complex with Arl3 of Chlamydomonas reinhardtii

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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