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5flz

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Revision as of 19:52, 10 April 2020

Cryo-EM structure of gamma-TuSC oligomers in a closed conformation

5flz, resolution 6.90Å

Structural highlights

5flz is a 6 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5fm1
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SPC97_YEAST] Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB. [TBG_YEAST] Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. TUB4 is an important spindle pole body component that organizes both cytoplasmic and nuclear microtubule arrays. [SPC98_YEAST] Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB.

Publication Abstract from PubMed

Modeling protein complex structures based on distantly related homologues can be challenging due to poor sequence and structure conservation. Therefore, utilizing even low-resolution experimental data can significantly increase model precision and accuracy. Here, we present models of the two key functional states of the yeast gamma-tubulin small complex (gammaTuSC): one for the low-activity "open" state and another for the higher-activity "closed" state. Both models were computed based on remotely related template structures and cryo-EM density maps at 6.9A and 8.0A resolution, respectively. For each state, extensive sampling of alignments and conformations was guided by the fit to the corresponding cryo-EM density map. The resulting good-scoring models formed a tightly clustered ensemble of conformations in most regions. We found significant structural differences between the two states, primarily in the gamma-tubulin subunit regions where the microtubule binds. We also report a set of chemical cross-links that were found to be consistent with equilibrium between the open and closed states. The protocols developed here have been incorporated into our open-source Integrative Modeling Platform (IMP) software package (http://integrativemodeling.org), and can therefore be applied to many other systems.

Structure of gamma-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure.,Greenberg CH, Kollman J, Zelter A, Johnson R, MacCoss MJ, Davis TN, Agard DA, Sali A J Struct Biol. 2016 Jun;194(3):303-10. doi: 10.1016/j.jsb.2016.03.006. Epub 2016 , Mar 8. PMID:26968363^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Greenberg CH, Kollman J, Zelter A, Johnson R, MacCoss MJ, Davis TN, Agard DA, Sali A. Structure of gamma-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure. J Struct Biol. 2016 Jun;194(3):303-10. doi: 10.1016/j.jsb.2016.03.006. Epub 2016 , Mar 8. PMID:26968363 doi:http://dx.doi.org/10.1016/j.jsb.2016.03.006

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5flz"

@@ Line 3: / Line 3: @@
 <SX load='5flz' size='340' side='right' viewer='molstar' caption='[[5flz]], [[Resolution|resolution]] 6.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5flz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FLZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5flz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FLZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FLZ FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fm1|5fm1]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5flz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5flz OCA], [http://pdbe.org/5flz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5flz RCSB], [http://www.ebi.ac.uk/pdbsum/5flz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5flz ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5flz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5flz OCA], [http://pdbe.org/5flz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5flz RCSB], [http://www.ebi.ac.uk/pdbsum/5flz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5flz ProSAT]</span></td></tr>
 </table>
 == Function ==

5flz

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Revision as of 19:52, 10 April 2020

Cryo-EM structure of gamma-TuSC oligomers in a closed conformation

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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