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6j9f

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Revision as of 02:04, 11 April 2020

Cryo-EM structure of Xanthomonos oryzae transcription elongation complex with the bacteriophage protein P7

6j9f, resolution 3.95Å

Structural highlights

6j9f is a 9 chain structure with sequence from Xanom, Xanoo, Xanop and Xanthomonas campestris phage xp10. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	rpoA (XANOP), rpoB, XOO3394 (XANOM), rpoC (XANOP), rpoZ (XANOO)
Activity:	DNA-directed RNA polymerase, with EC number 2.7.7.6
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPOC_XANOP] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [RPOB_XANOM] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [RPOA_XANOP] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [A0A0U4VN94_XANOO] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.[HAMAP-Rule:MF_00366][SAAS:SAAS00387808]

Publication Abstract from PubMed

Bacteriophages typically hijack the host bacterial transcriptional machinery to regulate their own gene expression and that of the host bacteria. The structural basis for bacteriophage protein-mediated transcription regulation-in particular transcription antitermination-is largely unknown. Here we report the 3.4 A and 4.0 A cryo-EM structures of two bacterial transcription elongation complexes (P7-NusA-TEC and P7-TEC) comprising the bacteriophage protein P7, a master host-transcription regulator encoded by bacteriophage Xp10 of the rice pathogen Xanthomonas oryzae pv. Oryzae (Xoo) and discuss the mechanisms by which P7 modulates the host bacterial RNAP. The structures together with biochemical evidence demonstrate that P7 prevents transcription termination by plugging up the RNAP RNA-exit channel and impeding RNA-hairpin formation at the intrinsic terminator. Moreover, P7 inhibits transcription initiation by restraining RNAP-clamp motions. Our study reveals the structural basis for transcription antitermination by phage proteins and provides insights into bacterial transcription regulation.

Structural basis for transcription antitermination at bacterial intrinsic terminator.,You L, Shi J, Shen L, Li L, Fang C, Yu C, Cheng W, Feng Y, Zhang Y Nat Commun. 2019 Jul 11;10(1):3048. doi: 10.1038/s41467-019-10955-x. PMID:31296855^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ You L, Shi J, Shen L, Li L, Fang C, Yu C, Cheng W, Feng Y, Zhang Y. Structural basis for transcription antitermination at bacterial intrinsic terminator. Nat Commun. 2019 Jul 11;10(1):3048. doi: 10.1038/s41467-019-10955-x. PMID:31296855 doi:http://dx.doi.org/10.1038/s41467-019-10955-x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6j9f"

@@ Line 3: / Line 3: @@
 <SX load='6j9f' size='340' side='right' viewer='molstar' caption='[[6j9f]], [[Resolution|resolution]] 3.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6j9f]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanom Xanom], [http://en.wikipedia.org/wiki/Xanoo Xanoo], [http://en.wikipedia.org/wiki/Xanop Xanop] and [http://en.wikipedia.org/wiki/Xanthomonas_campestris_phage_xp10 Xanthomonas campestris phage xp10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J9F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J9F FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6j9f]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanom Xanom], [http://en.wikipedia.org/wiki/Xanoo Xanoo], [http://en.wikipedia.org/wiki/Xanop Xanop] and [http://en.wikipedia.org/wiki/Xanthomonas_campestris_phage_xp10 Xanthomonas campestris phage xp10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J9F OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6J9F FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpoA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360094 XANOP]), rpoB, XOO3394 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342109 XANOM]), rpoC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360094 XANOP]), rpoZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64187 XANOO])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j9f OCA], [http://pdbe.org/6j9f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j9f RCSB], [http://www.ebi.ac.uk/pdbsum/6j9f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j9f ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6j9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j9f OCA], [http://pdbe.org/6j9f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j9f RCSB], [http://www.ebi.ac.uk/pdbsum/6j9f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j9f ProSAT]</span></td></tr>
 </table>
 == Function ==

6j9f

From Proteopedia

Revision as of 02:04, 11 April 2020

Cryo-EM structure of Xanthomonos oryzae transcription elongation complex with the bacteriophage protein P7

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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