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6mhy
From Proteopedia
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==Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM== | ==Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM== | ||
| - | < | + | <SX load='6mhy' size='340' side='right' viewer='molstar' caption='[[6mhy]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6mhy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MHY OCA]. For a <b>guided tour on the structure components</b> use [http:// | + | <table><tr><td colspan='2'>[[6mhy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MHY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MHY FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mhy OCA], [http://pdbe.org/6mhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mhy RCSB], [http://www.ebi.ac.uk/pdbsum/6mhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mhy ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/CXA8_SHEEP CXA8_SHEEP]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. | [[http://www.uniprot.org/uniprot/CXA8_SHEEP CXA8_SHEEP]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Gap junctions establish direct pathways for cell-to-cell communication through the assembly of twelve connexin subunits that form intercellular channels connecting neighbouring cells. Co-assembly of different connexin isoforms produces channels with unique properties and enables communication across cell types. Here we used single-particle cryo-electron microscopy to investigate the structural basis of connexin co-assembly in native lens gap junction channels composed of connexin 46 and connexin 50 (Cx46/50). We provide the first comparative analysis to connexin 26 (Cx26), which-together with computational studies-elucidates key energetic features governing gap junction permselectivity. Cx46/50 adopts an open-state conformation that is distinct from the Cx26 crystal structure, yet it appears to be stabilized by a conserved set of hydrophobic anchoring residues. 'Hot spots' of genetic mutations linked to hereditary cataract formation map to the core structural-functional elements identified in Cx46/50, suggesting explanations for many of the disease-causing effects. | ||
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| + | Structure of native lens connexin 46/50 intercellular channels by cryo-EM.,Myers JB, Haddad BG, O'Neill SE, Chorev DS, Yoshioka CC, Robinson CV, Zuckerman DM, Reichow SL Nature. 2018 Dec;564(7736):372-377. doi: 10.1038/s41586-018-0786-7. Epub 2018 Dec, 12. PMID:30542154<ref>PMID:30542154</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6mhy" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Connexin 3D structure|Connexin 3D structure]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
| + | [[Category: Large Structures]] | ||
[[Category: Ovis aries]] | [[Category: Ovis aries]] | ||
[[Category: Myers, J B]] | [[Category: Myers, J B]] | ||
Current revision
Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM
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