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6mhy

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Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM

6mhy, resolution 3.40Å

Structural highlights

6mhy is a 12 chain structure with sequence from Ovis aries. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CXA8_SHEEP] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Publication Abstract from PubMed

Gap junctions establish direct pathways for cell-to-cell communication through the assembly of twelve connexin subunits that form intercellular channels connecting neighbouring cells. Co-assembly of different connexin isoforms produces channels with unique properties and enables communication across cell types. Here we used single-particle cryo-electron microscopy to investigate the structural basis of connexin co-assembly in native lens gap junction channels composed of connexin 46 and connexin 50 (Cx46/50). We provide the first comparative analysis to connexin 26 (Cx26), which-together with computational studies-elucidates key energetic features governing gap junction permselectivity. Cx46/50 adopts an open-state conformation that is distinct from the Cx26 crystal structure, yet it appears to be stabilized by a conserved set of hydrophobic anchoring residues. 'Hot spots' of genetic mutations linked to hereditary cataract formation map to the core structural-functional elements identified in Cx46/50, suggesting explanations for many of the disease-causing effects.

Structure of native lens connexin 46/50 intercellular channels by cryo-EM.,Myers JB, Haddad BG, O'Neill SE, Chorev DS, Yoshioka CC, Robinson CV, Zuckerman DM, Reichow SL Nature. 2018 Dec;564(7736):372-377. doi: 10.1038/s41586-018-0786-7. Epub 2018 Dec, 12. PMID:30542154^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Myers JB, Haddad BG, O'Neill SE, Chorev DS, Yoshioka CC, Robinson CV, Zuckerman DM, Reichow SL. Structure of native lens connexin 46/50 intercellular channels by cryo-EM. Nature. 2018 Dec;564(7736):372-377. doi: 10.1038/s41586-018-0786-7. Epub 2018 Dec, 12. PMID:30542154 doi:http://dx.doi.org/10.1038/s41586-018-0786-7

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6mhy"

@@ Line 1: / Line 1: @@
 ==Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM==
-<StructureSection load='6mhy' size='340' side='right'caption='[[6mhy]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+<SX load='6mhy' size='340' side='right' viewer='molstar' caption='[[6mhy]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6mhy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MHY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6mhy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MHY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MHY FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mhy OCA], [http://pdbe.org/6mhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mhy RCSB], [http://www.ebi.ac.uk/pdbsum/6mhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mhy ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mhy OCA], [http://pdbe.org/6mhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mhy RCSB], [http://www.ebi.ac.uk/pdbsum/6mhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mhy ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Large Structures]]
 [[Category: Ovis aries]]

6mhy

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Current revision

Structure of connexin-50 intercellular gap junction channel at 3.4 angstrom resolution by cryoEM

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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