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6nbb
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6nbb is ON HOLD Authors: Herzik Jr., M.A., Wu, M., Lander, G.C. Description: Horse liver alcohol dehydrogenase determined using single-particle cry...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Horse liver alcohol dehydrogenase determined using single-particle cryo-EM at 200 keV== | |
| + | <SX load='6nbb' size='340' side='right' viewer='molstar' caption='[[6nbb]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6nbb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NBB FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbb OCA], [http://pdbe.org/6nbb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nbb RCSB], [http://www.ebi.ac.uk/pdbsum/6nbb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Determining high-resolution structures of biological macromolecules amassing less than 100 kilodaltons (kDa) has been a longstanding goal of the cryo-electron microscopy (cryo-EM) community. While the Volta phase plate has enabled visualization of specimens in this size range, this instrumentation is not yet fully automated and can present technical challenges. Here, we show that conventional defocus-based cryo-EM methodologies can be used to determine high-resolution structures of specimens amassing less than 100 kDa using a transmission electron microscope operating at 200 keV coupled with a direct electron detector. Our ~2.7 A structure of alcohol dehydrogenase (82 kDa) proves that bound ligands can be resolved with high fidelity to enable investigation of drug-target interactions. Our ~2.8 A and ~3.2 A structures of methemoglobin demonstrate that distinct conformational states can be identified within a dataset for proteins as small as 64 kDa. Furthermore, we provide the sub-nanometer cryo-EM structure of a sub-50 kDa protein. | ||
| - | + | High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM.,Herzik MA Jr, Wu M, Lander GC Nat Commun. 2019 Mar 4;10(1):1032. doi: 10.1038/s41467-019-08991-8. PMID:30833564<ref>PMID:30833564</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6nbb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Alcohol dehydrogenase]] | ||
| + | [[Category: Equus caballus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Herzik, M A]] | ||
| + | [[Category: Lander, G C]] | ||
[[Category: Wu, M]] | [[Category: Wu, M]] | ||
| - | [[Category: | + | [[Category: Dehydrogenase]] |
| - | [[Category: | + | [[Category: Homo-2-mer]] |
| - | [[Category: | + | [[Category: Nadh-binding]] |
| + | [[Category: Oxidoreductase]] | ||
Current revision
Horse liver alcohol dehydrogenase determined using single-particle cryo-EM at 200 keV
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