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6oya
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6oya is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the Rhodopsin-Transducin-Nanobody Complex== | |
| + | <SX load='6oya' size='340' side='right' viewer='molstar' caption='[[6oya]], [[Resolution|resolution]] 3.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6oya]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin], [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OYA FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oya OCA], [http://pdbe.org/6oya PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oya RCSB], [http://www.ebi.ac.uk/pdbsum/6oya PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oya ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/GNAT1_BOVIN GNAT1_BOVIN]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. [[http://www.uniprot.org/uniprot/GBB1_BOVIN GBB1_BOVIN]] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. [[http://www.uniprot.org/uniprot/OPSD_BOVIN OPSD_BOVIN]] Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity).<ref>PMID:16908857</ref> <ref>PMID:17060607</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rhodopsin (Rho), a prototypical G-protein-coupled receptor (GPCR) in vertebrate vision, activates the G-protein transducin (GT) by catalyzing GDP-GTP exchange on its alpha subunit (GalphaT). To elucidate the determinants of GT coupling and activation, we obtained cryo-EM structures of a fully functional, light-activated Rho-GT complex in the presence and absence of a G-protein-stabilizing nanobody. The structures illustrate how GT overcomes its low basal activity by engaging activated Rho in a conformation distinct from other GPCR-G-protein complexes. Moreover, the nanobody-free structures reveal native conformations of G-protein components and capture three distinct conformers showing the GalphaT helical domain (alphaHD) contacting the Gbetagamma subunits. These findings uncover the molecular underpinnings of G-protein activation by visual rhodopsin and shed new light on the role played by Gbetagamma during receptor-catalyzed nucleotide exchange. | ||
| - | + | Structures of the Rhodopsin-Transducin Complex: Insights into G-Protein Activation.,Gao Y, Hu H, Ramachandran S, Erickson JW, Cerione RA, Skiniotis G Mol Cell. 2019 Jun 24. pii: S1097-2765(19)30440-X. doi:, 10.1016/j.molcel.2019.06.007. PMID:31300275<ref>PMID:31300275</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6oya" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Rhodopsin 3D structures|Rhodopsin 3D structures]] | ||
| + | *[[Transducin 3D structures|Transducin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Bos taurus]] | ||
| + | [[Category: Bovin]] | ||
| + | [[Category: Camelus glama]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cerione, R A]] | ||
| + | [[Category: Erickson, J W]] | ||
| + | [[Category: Gao, Y]] | ||
| + | [[Category: Hu, H]] | ||
| + | [[Category: Ramachandran, S]] | ||
| + | [[Category: Skiniotis, G]] | ||
| + | [[Category: Complex]] | ||
| + | [[Category: G protein]] | ||
| + | [[Category: Gpcr]] | ||
| + | [[Category: Signaling protein-immune system complex]] | ||
Current revision
Structure of the Rhodopsin-Transducin-Nanobody Complex
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