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6pza

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Cryo-EM structure of the pancreatic beta-cell SUR1 bound to ATP and glibenclamide

6pza, resolution 3.74Å

Structural highlights

6pza is a 2 chain structure with sequence from Black-bellied hamster and Buffalo rat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	6pzb, 6pz9, 6pzi, 6pzc
Gene:	ABCC8, SUR (Black-bellied hamster), Kcnj11 (Buffalo rat)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ABCC8_CRICR] Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive potassium channels and insulin release.[UniProtKB:Q09428] [KCJ11_RAT] This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).

Publication Abstract from PubMed

ATP-sensitive potassium (KATP) channels composed of a pore-forming Kir6.2 potassium channel and a regulatory ABC transporter sulfonylurea receptor 1 (SUR1) regulate insulin secretion in pancreatic beta-cells to maintain glucose homeostasis. Mutations that impair channel folding or assembly prevent cell surface expression and cause congenital hyperinsulinism. Structurally diverse KATP inhibitors are known to act as pharmacochaperones to correct mutant channel expression, but the mechanism is unknown. Here, we compare cryoEM structures of a mammalian KATP channel bound to pharmacochaperones glibenclamide, repaglinide, and carbamazepine. We found all three drugs bind within a common pocket in SUR1. Further, we found the N-terminus of Kir6.2 inserted within the central cavity of the SUR1 ABC core, adjacent the drug binding pocket. The findings reveal a common mechanism by which diverse compounds stabilize the Kir6.2 N-terminus within SUR1's ABC core, allowing it to act as a firm 'handle' for the assembly of metastable mutant SUR1-Kir6.2 complexes.

Mechanism of pharmacochaperoning in a mammalian KATP channel revealed by cryo-EM.,Martin GM, Sung MW, Yang Z, Innes LM, Kandasamy B, David LL, Yoshioka C, Shyng SL Elife. 2019 Jul 25;8. pii: 46417. doi: 10.7554/eLife.46417. PMID:31343405^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Martin GM, Sung MW, Yang Z, Innes LM, Kandasamy B, David LL, Yoshioka C, Shyng SL. Mechanism of pharmacochaperoning in a mammalian KATP channel revealed by cryo-EM. Elife. 2019 Jul 25;8. pii: 46417. doi: 10.7554/eLife.46417. PMID:31343405 doi:http://dx.doi.org/10.7554/eLife.46417

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6pza"

@@ Line 1: / Line 1: @@
 ==Cryo-EM structure of the pancreatic beta-cell SUR1 bound to ATP and glibenclamide==
-<StructureSection load='6pza' size='340' side='right'caption='[[6pza]], [[Resolution|resolution]] 3.74&Aring;' scene=''>
+<SX load='6pza' size='340' side='right' viewer='molstar' caption='[[6pza]], [[Resolution|resolution]] 3.74&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6pza]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-bellied_hamster Black-bellied hamster] and [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PZA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6pza]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Black-bellied_hamster Black-bellied hamster] and [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PZA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6PZA FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GBM:5-CHLORO-N-(2-{4-[(CYCLOHEXYLCARBAMOYL)SULFAMOYL]PHENYL}ETHYL)-2-METHOXYBENZAMIDE'>GBM</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GBM:5-CHLORO-N-(2-{4-[(CYCLOHEXYLCARBAMOYL)SULFAMOYL]PHENYL}ETHYL)-2-METHOXYBENZAMIDE'>GBM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6pzb|6pzb]], [[6pz9|6pz9]], [[6pzi|6pzi]], [[6pzc|6pzc]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ABCC8, SUR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10034 Black-bellied hamster]), Kcnj11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pza OCA], [http://pdbe.org/6pza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pza RCSB], [http://www.ebi.ac.uk/pdbsum/6pza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pza ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6pza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pza OCA], [http://pdbe.org/6pza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pza RCSB], [http://www.ebi.ac.uk/pdbsum/6pza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pza ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 26: / Line 26: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Black-bellied hamster]]
 [[Category: Buffalo rat]]

6pza

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Current revision

Cryo-EM structure of the pancreatic beta-cell SUR1 bound to ATP and glibenclamide

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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