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6q06
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The entry | + | ==MERS-CoV S structure in complex with 2,3-sialyl-N-acetyl-lactosamine== |
| + | <SX load='6q06' size='340' side='right' viewer='molstar' caption='[[6q06]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6q06]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_betacoronavirus_2c_emc/2012 Human betacoronavirus 2c emc/2012]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q06 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Q06 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FOL:FOLIC+ACID'>FOL</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6q06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q06 OCA], [http://pdbe.org/6q06 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q06 RCSB], [http://www.ebi.ac.uk/pdbsum/6q06 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q06 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe and often lethal respiratory illness in humans, and no vaccines or specific treatments are available. Infections are initiated via binding of the MERS-CoV spike (S) glycoprotein to sialosides and dipeptidyl-peptidase 4 (the attachment and entry receptors, respectively). To understand MERS-CoV engagement of sialylated receptors, we determined the cryo-EM structures of S in complex with 5-N-acetyl neuraminic acid, 5-N-glycolyl neuraminic acid, sialyl-Lewis(X), alpha2,3-sialyl-N-acetyl-lactosamine and alpha2,6-sialyl-N-acetyl-lactosamine at 2.7-3.0 A resolution. We show that recognition occurs via a conserved groove that is essential for MERS-CoV S-mediated attachment to sialosides and entry into human airway epithelial cells. Our data illuminate MERS-CoV S sialoside specificity and suggest that selectivity for alpha2,3-linked over alpha2,6-linked receptors results from enhanced interactions with the former class of oligosaccharides. This study provides a structural framework explaining MERS-CoV attachment to sialoside receptors and identifies a site of potential vulnerability to inhibitors of viral entry. | ||
| - | + | Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors.,Park YJ, Walls AC, Wang Z, Sauer MM, Li W, Tortorici MA, Bosch BJ, DiMaio F, Veesler D Nat Struct Mol Biol. 2019 Dec;26(12):1151-1157. doi: 10.1038/s41594-019-0334-7., Epub 2019 Dec 2. PMID:31792450<ref>PMID:31792450</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6q06" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Spike protein|Spike protein]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Human betacoronavirus 2c emc/2012]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bosch, B J]] | ||
| + | [[Category: DiMaio, F D]] | ||
| + | [[Category: Li, W]] | ||
| + | [[Category: Park, Y J]] | ||
| + | [[Category: Structural genomic]] | ||
| + | [[Category: Sauer, M]] | ||
| + | [[Category: Tortorici, M A]] | ||
| + | [[Category: Veesler, D]] | ||
| + | [[Category: Walls, A C]] | ||
| + | [[Category: Wang, Z]] | ||
| + | [[Category: Coronavirus]] | ||
| + | [[Category: Membrane fusion]] | ||
| + | [[Category: Mers-cov]] | ||
| + | [[Category: Spike glycoprotein]] | ||
| + | [[Category: Ssgcid]] | ||
| + | [[Category: Viral protein]] | ||
Current revision
MERS-CoV S structure in complex with 2,3-sialyl-N-acetyl-lactosamine
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