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6rwx

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Periplasmic inner membrane ring of the Shigella type 3 secretion system

6rwx, resolution 3.55Å

Structural highlights

6rwx is a 48 chain structure with sequence from Shigella flexneri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	6rwk, 6rwy
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MXIG_SHIFL] Involved in the secretion of the Ipa antigens. Involved in the intracellular dissemination of Shigella. Part of the Mxi-Spa secretion apparatus. [MXIJ_SHIFL] Involved in the secretion of the Ipa antigens.

Publication Abstract from PubMed

The Type III Secretion Systems (T3SS) needle complex is a conserved syringe-shaped protein translocation nanomachine with a mass of about 3.5 MDa essential for the survival and virulence of many Gram-negative bacterial pathogens. This system is composed of a membrane-embedded basal body and an extracellular needle that deliver effector proteins into host cells. High-resolution structures of the T3SS from different organisms and infection stages are needed to understand the underlying molecular mechanisms of effector translocation. Here, we present the cryo-electron microscopy structure of the isolated Shigella T3SS needle complex. The inner membrane (IM) region of the basal body adopts 24-fold rotational symmetry and forms a channel system that connects the bacterial periplasm with the export apparatus cage. The secretin oligomer adopts a heterogeneous architecture with 16- and 15-fold cyclic symmetry in the periplasmic N-terminal connector and C-terminal outer membrane ring, respectively. Two out of three IM subunits bind the secretin connector via a beta-sheet augmentation. The cryo-EM map also reveals the helical architecture of the export apparatus core, the inner rod, the needle and their intervening interfaces.

Cryo-EM structure of the Shigella type III needle complex.,Lunelli M, Kamprad A, Burger J, Mielke T, Spahn CMT, Kolbe M PLoS Pathog. 2020 Feb 24;16(2):e1008263. doi: 10.1371/journal.ppat.1008263. PMID:32092125^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lunelli M, Kamprad A, Burger J, Mielke T, Spahn CMT, Kolbe M. Cryo-EM structure of the Shigella type III needle complex. PLoS Pathog. 2020 Feb 24;16(2):e1008263. doi: 10.1371/journal.ppat.1008263. PMID:32092125 doi:http://dx.doi.org/10.1371/journal.ppat.1008263

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6rwx"

@@ Line 1: / Line 1: @@
 ==Periplasmic inner membrane ring of the Shigella type 3 secretion system==
-<StructureSection load='6rwx' size='340' side='right'caption='[[6rwx]], [[Resolution|resolution]] 3.55&Aring;' scene=''>
+<SX load='6rwx' size='340' side='right' viewer='molstar' caption='[[6rwx]], [[Resolution|resolution]] 3.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6rwx]] is a 48 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RWX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6rwx]] is a 48 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RWX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6RWX FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6rwk|6rwk]], [[6rwy|6rwy]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rwx OCA], [http://pdbe.org/6rwx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rwx RCSB], [http://www.ebi.ac.uk/pdbsum/6rwx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rwx ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6rwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rwx OCA], [http://pdbe.org/6rwx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rwx RCSB], [http://www.ebi.ac.uk/pdbsum/6rwx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rwx ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Large Structures]]
 [[Category: Shigella flexneri]]

6rwx

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Periplasmic inner membrane ring of the Shigella type 3 secretion system

Structural highlights

Function

Publication Abstract from PubMed

References

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