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6rxu

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Revision as of 05:55, 11 April 2020

Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B1

6rxu, resolution 3.50Å

Structural highlights

6rxu is a 66 chain structure with sequence from Chaetomium thermophilum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[G0S3V7_CHATD] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[PIRNR:PIRNR006515] [PNO1_CHATD] Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.[UniProtKB:Q99216] [UTP11_CHATD] Involved in nucleolar processing of pre-18S ribosomal RNA.[UniProtKB:P34247] [NOL10_CHATD] May be involved in rRNA-processing and ribosome biosynthesis.[UniProtKB:P48234]

Publication Abstract from PubMed

Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.

Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.,Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E Mol Cell. 2019 Jul 15. pii: S1097-2765(19)30489-7. doi:, 10.1016/j.molcel.2019.06.032. PMID:31378463^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E. Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration. Mol Cell. 2019 Jul 15. pii: S1097-2765(19)30489-7. doi:, 10.1016/j.molcel.2019.06.032. PMID:31378463 doi:http://dx.doi.org/10.1016/j.molcel.2019.06.032

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6rxu"

@@ Line 3: / Line 3: @@
 <SX load='6rxu' size='340' side='right' viewer='molstar' caption='[[6rxu]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6rxu]] is a 66 chain structure with sequence from [http://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RXU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6rxu]] is a 66 chain structure with sequence from [http://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RXU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6RXU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxu OCA], [http://pdbe.org/6rxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rxu RCSB], [http://www.ebi.ac.uk/pdbsum/6rxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxu ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6rxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxu OCA], [http://pdbe.org/6rxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rxu RCSB], [http://www.ebi.ac.uk/pdbsum/6rxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxu ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/G0S3V7_CHATD G0S3V7_CHATD]] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[PIRNR:PIRNR006515] [[http://www.uniprot.org/uniprot/PNO1_CHATD PNO1_CHATD]] Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.[UniProtKB:Q99216] [[http://www.uniprot.org/uniprot/NOL10_CHATD NOL10_CHATD]] May be involved in rRNA-processing and ribosome biosynthesis.[UniProtKB:P48234] [[http://www.uniprot.org/uniprot/UTP11_CHATD UTP11_CHATD]] Involved in nucleolar processing of pre-18S ribosomal RNA.[UniProtKB:P34247]
+[[http://www.uniprot.org/uniprot/G0S3V7_CHATD G0S3V7_CHATD]] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[PIRNR:PIRNR006515] [[http://www.uniprot.org/uniprot/PNO1_CHATD PNO1_CHATD]] Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.[UniProtKB:Q99216] [[http://www.uniprot.org/uniprot/UTP11_CHATD UTP11_CHATD]] Involved in nucleolar processing of pre-18S ribosomal RNA.[UniProtKB:P34247] [[http://www.uniprot.org/uniprot/NOL10_CHATD NOL10_CHATD]] May be involved in rRNA-processing and ribosome biosynthesis.[UniProtKB:P48234]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

6rxu

From Proteopedia

Revision as of 05:55, 11 April 2020

Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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