1b0z
From Proteopedia
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'''The crystal structure of phosphoglucose isomerase-an enzyme with autocrine motility factor activity in tumor cells''' | '''The crystal structure of phosphoglucose isomerase-an enzyme with autocrine motility factor activity in tumor cells''' | ||
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[[Category: Meng, M.]] | [[Category: Meng, M.]] | ||
[[Category: Sun, Y J.]] | [[Category: Sun, Y J.]] | ||
| - | [[Category: | + | [[Category: Autocrinefactor]] |
| - | [[Category: | + | [[Category: Crystallography motility]] |
| - | [[Category: | + | [[Category: Neuroleukin]] |
| - | [[Category: | + | [[Category: Phosphoglucose isomerase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:56:18 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:56, 2 May 2008
The crystal structure of phosphoglucose isomerase-an enzyme with autocrine motility factor activity in tumor cells
Overview
Phosphoglucose isomerase catalyzes the reversible isomerization of glucose 6-phosphate to fructose 6-phosphate. In addition, phosphoglucose isomerase has been shown to have functions equivalent to neuroleukin, autocrine motility factor, and maturation factor. Here we present the crystal structures of phosphoglucose isomerase complexed with 5-phospho-D-arabinonate and N-bromoacetylethanolamine phosphate at 2.5- and 2.3-A resolution, respectively. The inhibitors bind to a region within the domains' interface and interact with a histidine residue (His(306)) from the other subunit. We also demonstrated that the inhibitors not only affect the enzymatic activity of phosphoglucose isomerase, but can also inhibit the autocrine motility factor-induced cell motility of CT-26 mouse colon tumor cells. These results indicate that the substrate and the receptor binding sites of phosphoglucose isomerase and autocrine motility factor are located within close proximity to each other. Based on these two complex structures, together with biological and biochemical results, we propose a possible isomerization mechanism for phosphoglucose isomerase.
About this Structure
1B0Z is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
The crystal structure of phosphoglucose isomerase/autocrine motility factor/neuroleukin complexed with its carbohydrate phosphate inhibitors suggests its substrate/receptor recognition., Chou CC, Sun YJ, Meng M, Hsiao CD, J Biol Chem. 2000 Jul 28;275(30):23154-60. PMID:10770936 Page seeded by OCA on Fri May 2 10:56:18 2008
