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6uen

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Revision as of 06:47, 11 April 2020

Cryo-EM structure of the respiratory syncytial virus RNA polymerase

6uen, resolution 3.67Å

Structural highlights

6uen is a 5 chain structure with sequence from Hrsv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	L, MZ07_64039gpL, MZ07_64040gpL (HRSV)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[G8EJ12_HRSV] Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular protein N concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals.[PIRNR:PIRNR000830]

Publication Abstract from PubMed

The respiratory syncytial virus (RSV) RNA polymerase, constituted of a 250 kDa large (L) protein and tetrameric phosphoprotein (P), catalyzes three distinct enzymatic activities - nucleotide polymerization, cap addition, and cap methylation. How RSV L and P coordinate these activities is poorly understood. Here, we present a 3.67 A cryo-EM structure of the RSV polymerase (L:P) complex. The structure reveals that the RNA dependent RNA polymerase (RdRp) and capping (Cap) domains of L interact with the oligomerization domain (POD) and C-terminal domain (PCTD) of a tetramer of P. The density of the methyltransferase (MT) domain of L and the N-terminal domain of P (PNTD) is missing. Further analysis and comparison with other RNA polymerases at different stages suggest the structure we obtained is likely to be at an elongation-compatible stage. Together, these data provide enriched insights into the interrelationship, the inhibitors, and the evolutionary implications of the RSV polymerase.

Cryo-EM structure of the respiratory syncytial virus RNA polymerase.,Cao D, Gao Y, Roesler C, Rice S, D'Cunha P, Zhuang L, Slack J, Domke M, Antonova A, Romanelli S, Keating S, Forero G, Juneja P, Liang B Nat Commun. 2020 Jan 17;11(1):368. doi: 10.1038/s41467-019-14246-3. PMID:31953395^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cao D, Gao Y, Roesler C, Rice S, D'Cunha P, Zhuang L, Slack J, Domke M, Antonova A, Romanelli S, Keating S, Forero G, Juneja P, Liang B. Cryo-EM structure of the respiratory syncytial virus RNA polymerase. Nat Commun. 2020 Jan 17;11(1):368. doi: 10.1038/s41467-019-14246-3. PMID:31953395 doi:http://dx.doi.org/10.1038/s41467-019-14246-3

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6uen"

@@ Line 3: / Line 3: @@
 <SX load='6uen' size='340' side='right' viewer='molstar' caption='[[6uen]], [[Resolution|resolution]] 3.67&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6uen]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Hrsv Hrsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UEN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6uen]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Hrsv Hrsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UEN FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L, MZ07_64039gpL, MZ07_64040gpL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11250 HRSV])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uen OCA], [http://pdbe.org/6uen PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uen RCSB], [http://www.ebi.ac.uk/pdbsum/6uen PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uen ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uen OCA], [http://pdbe.org/6uen PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uen RCSB], [http://www.ebi.ac.uk/pdbsum/6uen PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uen ProSAT]</span></td></tr>
 </table>
 == Function ==

6uen

From Proteopedia

Revision as of 06:47, 11 April 2020

Cryo-EM structure of the respiratory syncytial virus RNA polymerase

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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