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Tailspike protein
From Proteopedia
(Difference between revisions)
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| - | <StructureSection load=' | + | <StructureSection load='3th0' size='340' side='right' caption='Tailspike protein from bacteriophage P222 complex with octasaccharide (PDB code [[3th0]])' scene=''> |
== Function == | == Function == | ||
| - | '''Tailspike protein''' (TSP) of bacteriophage P22 is a trimeric protein which serves as the receptor-binding protein of the bacteriophage to its bacterial host<ref>PMID:29500571</ref>. | + | '''Tailspike protein''' (TSP) of bacteriophage P22 is a trimeric protein which serves as the receptor-binding protein of the bacteriophage to its bacterial host<ref>PMID:29500571</ref>. O-antigen polysaccharides are the natural substrates for TSP<ref>PMID:20817910</ref>. |
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== Relevance == | == Relevance == | ||
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| + | Bacteriophage research is becoming important due to antibiotics resistance. TSP binding to pathogenic bacteria can be used for their detection<ref>PMID:30111705</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
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**[[1tyv]], [[1tyu]], [[1tyx]], [[1tyw]], [[2vfm]], [[1tsp]] - P22TSP CTD <br /> | **[[1tyv]], [[1tyu]], [[1tyx]], [[1tyw]], [[2vfm]], [[1tsp]] - P22TSP CTD <br /> | ||
| - | **[[1qq1]], [[1qrc]], [[1qrb]], [[2vfp]], [[2vfo]], [[2vfq]], [[1qa3]], [[1qa2]], [[1qa1]], [[2vfn]], [[1clw]] - P22TSP CTD | + | **[[1qq1]], [[1qrc]], [[1qrb]], [[2vfp]], [[2vfo]], [[2vfq]], [[1qa3]], [[1qa2]], [[1qa1]], [[2vfn]], [[1clw]] - P22TSP CTD (mutant)<br /> |
| - | + | ||
**[[4avz]], [[4xon]], [[4xqf]], [[4xlc]], [[4xl9]], [[4xkv]], [[4xlh]], [[4xnf]], [[4xn0]] - HK520TSP CTD (mutant)<br /> | **[[4avz]], [[4xon]], [[4xqf]], [[4xlc]], [[4xl9]], [[4xkv]], [[4xlh]], [[4xnf]], [[4xn0]] - HK520TSP CTD (mutant)<br /> | ||
| - | **[[2x85]], [[2vjj]], [[2x6x]], [[6g0x]] - HK520TSP CTD + polysaccharide<br /> | ||
| - | **[[2x6w]], [[2x6y]], [[4xla]], [[5xop]], [[5xor]], [[4xr6]], [[4yej]], [[4yel]], [[4xle]], [[4xlf]], [[4xkw]], [[4xm3]], [[4xmy]], [[4xn3]], [[4xot]], [[6gvp]], [[6gvr]] - HK520TSP CTD (mutant) + polysaccharide<br /> | ||
**[[2vbe]] - TSP CTD - bacteriophage SFVI<br /> | **[[2vbe]] - TSP CTD - bacteriophage SFVI<br /> | ||
**[[2vbk]] - SF6TSP CTD - bacteriophage SF6<br /> | **[[2vbk]] - SF6TSP CTD - bacteriophage SF6<br /> | ||
| - | **[[2vbm]], [[4urr]] - SF6TSP CTD + polysaccharide<br /> | ||
**[[5w6h]] - CBA120TSP4 active domain 344-1056<br /> | **[[5w6h]] - CBA120TSP4 active domain 344-1056<br /> | ||
| - | **[[5w6s]] - CBA120TSP4 active domain + polysaccharide<br /> | ||
**[[6f7d]], [[6f7k]], [[2v5i]] - SF6TSP CTD - bacteriophage DET7<br />> | **[[6f7d]], [[6f7k]], [[2v5i]] - SF6TSP CTD - bacteriophage DET7<br />> | ||
**[[6e0v]] - PHI92TSP CTD - bacteriophage PHI92<br /> | **[[6e0v]] - PHI92TSP CTD - bacteriophage PHI92<br /> | ||
| - | **[[6e0w]] - PHI92TSP CTD + colanic acid<br /> | ||
**[[6e1r]] - TSP CTD - bacteriophage VB_APIP_P1<br /> | **[[6e1r]] - TSP CTD - bacteriophage VB_APIP_P1<br /> | ||
**[[6eu4]] - TSP CTD - bacteriophage VB_ABAP_AS12<br /> | **[[6eu4]] - TSP CTD - bacteriophage VB_ABAP_AS12<br /> | ||
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**[[3gvj]], [[3gvk]] - KIFTSP CTD (mutant)<br /> | **[[3gvj]], [[3gvk]] - KIFTSP CTD (mutant)<br /> | ||
**[[3riq]] - TSP CTD - siphovirus 9NA<br /> | **[[3riq]] - TSP CTD - siphovirus 9NA<br /> | ||
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| + | *Tailspike protein CTD complex with polysaccharides | ||
| + | |||
| + | **[[3th0]] - P22TSP CTD + octasaccharide<br /> | ||
| + | **[[2x85]], [[2vjj]], [[2x6x]], [[6g0x]] - HK520TSP CTD + polysaccharide<br /> | ||
| + | **[[2x6w]], [[2x6y]], [[4xla]], [[5xop]], [[5xor]], [[4xr6]], [[4yej]], [[4yel]], [[4xle]], [[4xlf]], [[4xkw]], [[4xm3]], [[4xmy]], [[4xn3]], [[4xot]], [[6gvp]], [[6gvr]] - HK520TSP CTD (mutant) + polysaccharide<br /> | ||
| + | **[[2vbm]], [[4urr]] - SF6TSP CTD + polysaccharide<br /> | ||
| + | **[[5w6s]] - CBA120TSP4 active domain + polysaccharide<br /> | ||
| + | **[[5w6s]] - CBA120TSP4 active domain + polysaccharide<br /> | ||
| + | **[[6e0w]] - PHI92TSP CTD + colanic acid<br /> | ||
}} | }} | ||
Revision as of 08:29, 11 April 2020
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3D structures of tailspike protein
Updated on 11-April-2020
References
- ↑ Williams J, Venkatesan K, Ayariga JA, Jackson D, Wu H, Villafane R. A genetic analysis of an important hydrophobic interaction at the P22 tailspike protein N-terminal domain. Arch Virol. 2018 Jun;163(6):1623-1633. doi: 10.1007/s00705-018-3777-y. Epub 2018 , Mar 2. PMID:29500571 doi:http://dx.doi.org/10.1007/s00705-018-3777-y
- ↑ Andres D, Hanke C, Baxa U, Seul A, Barbirz S, Seckler R. Tailspike interactions with lipopolysaccharide effect DNA ejection from phage P22 particles in vitro. J Biol Chem. 2010 Nov 19;285(47):36768-75. doi: 10.1074/jbc.M110.169003. Epub, 2010 Sep 3. PMID:20817910 doi:http://dx.doi.org/10.1074/jbc.M110.169003
- ↑ Kunstmann S, Scheidt T, Buchwald S, Helm A, Mulard LA, Fruth A, Barbirz S. Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens. Viruses. 2018 Aug 15;10(8). pii: v10080431. doi: 10.3390/v10080431. PMID:30111705 doi:http://dx.doi.org/10.3390/v10080431
