1b2c

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[[Image:1b2c.gif|left|200px]]
[[Image:1b2c.gif|left|200px]]
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{{Structure
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|PDB= 1b2c |SIZE=350|CAPTION= <scene name='initialview01'>1b2c</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1b2c", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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{{STRUCTURE_1b2c| PDB=1b2c | SCENE= }}
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|RELATEDENTRY=[[1b17|1B17]], [[1b18|1B18]], [[1b19|1B19]], [[1b2a|1B2A]], [[1b2b|1B2B]], [[1b2c|1B2C]], [[1b2d|1B2D]], [[1b2e|1B2E]], [[1b2f|1B2F]], [[1b2g|1B2G]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2c OCA], [http://www.ebi.ac.uk/pdbsum/1b2c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1b2c RCSB]</span>
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}}
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'''PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.26 COORDINATES)'''
'''PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.26 COORDINATES)'''
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[[Category: Caspar, D L.D.]]
[[Category: Caspar, D L.D.]]
[[Category: Diao, J S.]]
[[Category: Diao, J S.]]
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[[Category: hormone]]
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[[Category: Hormone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:58:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:52:04 2008''
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Revision as of 07:58, 2 May 2008

Image:1b2c.gif

Template:STRUCTURE 1b2c

PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.26 COORDINATES)


Overview

Structures of porcine insulin crystals soaked in 1 M sodium sulfate at pH 5.00, 5.53, 5.80, 6.00, 6.16, 6.26, 6.35, 6.50, 6.98 and 9.00 have been determined at between 1.7 and 1.9 A resolution. GluB13 exhibits a single conformation at pH </= 5.80, two conformations between pH 6.00 and 6.98 and a single conformation at pH 9.00. Between pH 6.00 and 6.98, the conformation of GluB13 switches from one rotamer to another rotamer. Between pH 6.16 and 6.26, PheB1 undergoes a significant conformational change. By pH 9.00 many residues have undergone relatively large shifts and HisB10 exhibits a double conformation. As a result of the pH increase, the occupancy of the sulfate ion decreases from a maximum of 1.00 at pH 5.00 to a minimum of 0.46 at pH 6.50. Comparison of the structures, the observed and calculated structure factors and map correlation coefficients indicate that the porcine insulin structure changes gradually as a function of pH.

About this Structure

1B2C is a Protein complex structure of sequences from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Crystallographic titration of cubic insulin crystals: pH affects GluB13 switching and sulfate binding., Diao J, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):670-6. Epub 2003, Mar 25. PMID:12657786 Page seeded by OCA on Fri May 2 10:58:30 2008

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