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6tg9
From Proteopedia
(Difference between revisions)
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==Cryo-EM Structure of NADH reduced form of NAD+-dependent Formate Dehydrogenase from Rhodobacter capsulatus== | ==Cryo-EM Structure of NADH reduced form of NAD+-dependent Formate Dehydrogenase from Rhodobacter capsulatus== | ||
| - | <StructureSection load='6tg9' size='340' side='right'caption='[[6tg9]]' scene=''> | + | <StructureSection load='6tg9' size='340' side='right'caption='[[6tg9]], [[Resolution|resolution]] 3.24Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TG9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TG9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6tg9]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus_y262 Rhodobacter capsulatus y262]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TG9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TG9 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tg9 OCA], [http://pdbe.org/6tg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tg9 RCSB], [http://www.ebi.ac.uk/pdbsum/6tg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tg9 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MO:MOLYBDENUM(VI)+ION'>6MO</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">U715_16550 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1415161 Rhodobacter capsulatus Y262]), U715_16555 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1415161 Rhodobacter capsulatus Y262]), U715_16560 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1415161 Rhodobacter capsulatus Y262]), U715_16540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1415161 Rhodobacter capsulatus Y262])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tg9 OCA], [http://pdbe.org/6tg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tg9 RCSB], [http://www.ebi.ac.uk/pdbsum/6tg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tg9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Metal-containing formate dehydrogenases (FDH) catalyse the reversible oxidation of formate to carbon dioxide at their molybdenum or tungsten active site. They display a diverse subunit and cofactor composition, but structural information on these enzymes is limited. Here we report the cryo-electron microscopic structures of the soluble Rhodobacter capsulatus FDH (RcFDH) as isolated and in the presence of reduced nicotinamide adenine dinucleotide (NADH). RcFDH assembles into a 360 kDa dimer of heterotetramers revealing a putative interconnection of electron pathway chains. In the presence of NADH, the RcFDH structure shows charging of cofactors, indicative of an increased electron load. | ||
| + | |||
| + | Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase.,Radon C, Mittelstadt G, Duffus BR, Burger J, Hartmann T, Mielke T, Teutloff C, Leimkuhler S, Wendler P Nat Commun. 2020 Apr 20;11(1):1912. doi: 10.1038/s41467-020-15614-0. PMID:32313256<ref>PMID:32313256</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6tg9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Formate dehydrogenase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Mittelstaedt G]] | + | [[Category: Rhodobacter capsulatus y262]] |
| - | [[Category: Radon C]] | + | [[Category: Mittelstaedt, G]] |
| - | [[Category: Wendler P]] | + | [[Category: Radon, C]] |
| + | [[Category: Wendler, P]] | ||
| + | [[Category: Formate oxidation]] | ||
| + | [[Category: Molybdoenzyme]] | ||
| + | [[Category: Nad+-dependent]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 07:08, 29 April 2020
Cryo-EM Structure of NADH reduced form of NAD+-dependent Formate Dehydrogenase from Rhodobacter capsulatus
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