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5fd3

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Revision as of 07:13, 6 May 2020

Structure of Lin54 tesmin domain bound to DNA

Structural highlights

5fd3 is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LIN54_HUMAN] Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner.

Publication Abstract from PubMed

The MuvB complex recruits transcription factors to activate or repress genes with cell cycle-dependent expression patterns. MuvB contains the DNA-binding protein LIN54, which directs the complex to promoter cell cycle genes homology region (CHR) elements. Here we characterize the DNA-binding properties of LIN54 and describe the structural basis for recognition of a CHR sequence. We biochemically define the CHR consensus as TTYRAA and determine that two tandem cysteine rich regions are required for high-affinity DNA association. A crystal structure of the LIN54 DNA-binding domain in complex with a CHR sequence reveals that sequence specificity is conferred by two tyrosine residues, which insert into the minor groove of the DNA duplex. We demonstrate that this unique tyrosine-mediated DNA binding is necessary for MuvB recruitment to target promoters. Our results suggest a model in which MuvB binds near transcription start sites and plays a role in positioning downstream nucleosomes.

Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters.,Marceau AH, Felthousen JG, Goetsch PD, Iness AN, Lee HW, Tripathi SM, Strome S, Litovchick L, Rubin SM Nat Commun. 2016 Jul 28;7:12301. doi: 10.1038/ncomms12301. PMID:27465258^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Marceau AH, Felthousen JG, Goetsch PD, Iness AN, Lee HW, Tripathi SM, Strome S, Litovchick L, Rubin SM. Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters. Nat Commun. 2016 Jul 28;7:12301. doi: 10.1038/ncomms12301. PMID:27465258 doi:http://dx.doi.org/10.1038/ncomms12301

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5fd3"

@@ Line 1: / Line 1: @@
 ==Structure of Lin54 tesmin domain bound to DNA==
-<StructureSection load='5fd3' size='340' side='right' caption='[[5fd3]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
+<StructureSection load='5fd3' size='340' side='right'caption='[[5fd3]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5fd3]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FD3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5fd3]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FD3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fd3 OCA], [http://pdbe.org/5fd3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fd3 RCSB], [http://www.ebi.ac.uk/pdbsum/5fd3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fd3 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fd3 OCA], [http://pdbe.org/5fd3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fd3 RCSB], [http://www.ebi.ac.uk/pdbsum/5fd3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fd3 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Felthousen, J G]]
 [[Category: Goetsch, P D]]

5fd3

From Proteopedia

Revision as of 07:13, 6 May 2020

Structure of Lin54 tesmin domain bound to DNA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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