5h2c

From Proteopedia

(Difference between revisions)

Revision as of 06:50, 20 May 2020

Crystal structure of Saccharomyces cerevisiae Osh1 ANK - Nvj1

Structural highlights

5h2c is a 2 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5h2d, 5h28, 5h2a
Gene:	SWH1, OSH1, YAR042W, YAR044W (Baker's yeast), NVJ1, VAB36, YHR195W (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[OSH1_YEAST] Lipid-binding protein involved in maintenance of intracellular sterol distribution and homeostasis. Binds to phosphoinositides. May be involved in formation of PMN vesicles by altering the membrane lipid composition.^[1] [NVJ1_YEAST] Involved in the formation of nucleus-vacuole (NV) junctions during piecemeal microautophagy of the nucleus (PMN). NV junctions are interorganelle interfaces mediated by NVJ1 in the nuclear envelope and VAC8 on the vacuole membrane. Together, NVJ1 and VAC8 form Velcro-like patches through which teardrop-like portions of the nucleus are pinched off into the vacuolar lumen and degraded by the PMN process. Acts also as an outer-nuclear membrane receptor for OSH1 and TSC13.^[2] ^[3] ^[4] ^[5] ^[6]

Publication Abstract from PubMed

Yeast Osh1 belongs to the oxysterol-binding protein (OSBP) family of proteins and contains multiple targeting modules optimized for lipid transport at the nucleus-vacuole junction (NVJ). The key determinants for NVJ targeting and the role of Osh1 at NVJs have remained elusive because of unknown lipid specificities. In this study, we determined the structures of the ankyrin repeat domain (ANK), and OSBP-related domain (ORD) of Osh1, in complex with Nvj1 and ergosterol, respectively. The Osh1 ANK forms a unique bi-lobed structure that recognizes a cytosolic helical segment of Nvj1. We discovered that Osh1 ORD binds ergosterol and phosphatidylinositol 4-phosphate PI(4)P in a competitive manner, suggesting counter-transport function of the two lipids. Ergosterol is bound to the hydrophobic pocket in a head-down orientation, and the structure of the PI(4)P-binding site in Osh1 is well conserved. Our results suggest that Osh1 performs non-vesicular transport of ergosterol and PI(4)P at the NVJ.

Structure of Yeast OSBP-Related Protein Osh1 Reveals Key Determinants for Lipid Transport and Protein Targeting at the Nucleus-Vacuole Junction.,Manik MK, Yang H, Tong J, Im YJ Structure. 2017 Apr 4;25(4):617-629.e3. doi: 10.1016/j.str.2017.02.010. Epub 2017, Mar 16. PMID:28319008^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Beh CT, Rine J. A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution. J Cell Sci. 2004 Jun 15;117(Pt 14):2983-96. Epub 2004 Jun 1. PMID:15173322 doi:http://dx.doi.org/10.1242/jcs.01157
↑ Pan X, Roberts P, Chen Y, Kvam E, Shulga N, Huang K, Lemmon S, Goldfarb DS. Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p. Mol Biol Cell. 2000 Jul;11(7):2445-57. PMID:10888680
↑ Roberts P, Moshitch-Moshkovitz S, Kvam E, O'Toole E, Winey M, Goldfarb DS. Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae. Mol Biol Cell. 2003 Jan;14(1):129-41. PMID:12529432 doi:http://dx.doi.org/10.1091/mbc.E02-08-0483
↑ Kvam E, Goldfarb DS. Nvj1p is the outer-nuclear-membrane receptor for oxysterol-binding protein homolog Osh1p in Saccharomyces cerevisiae. J Cell Sci. 2004 Oct 1;117(Pt 21):4959-68. Epub 2004 Sep 14. PMID:15367582 doi:http://dx.doi.org/10.1242/jcs.01372
↑ Kvam E, Gable K, Dunn TM, Goldfarb DS. Targeting of Tsc13p to nucleus-vacuole junctions: a role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles. Mol Biol Cell. 2005 Sep;16(9):3987-98. Epub 2005 Jun 15. PMID:15958487 doi:http://dx.doi.org/10.1091/mbc.E05-04-0290
↑ Kvam E, Goldfarb DS. Structure and function of nucleus-vacuole junctions: outer-nuclear-membrane targeting of Nvj1p and a role in tryptophan uptake. J Cell Sci. 2006 Sep 1;119(Pt 17):3622-33. Epub 2006 Aug 15. PMID:16912077 doi:http://dx.doi.org/10.1242/jcs.03093
↑ Manik MK, Yang H, Tong J, Im YJ. Structure of Yeast OSBP-Related Protein Osh1 Reveals Key Determinants for Lipid Transport and Protein Targeting at the Nucleus-Vacuole Junction. Structure. 2017 Apr 4;25(4):617-629.e3. doi: 10.1016/j.str.2017.02.010. Epub 2017, Mar 16. PMID:28319008 doi:http://dx.doi.org/10.1016/j.str.2017.02.010

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5h2c"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Saccharomyces cerevisiae Osh1 ANK - Nvj1==
-<StructureSection load='5h2c' size='340' side='right' caption='[[5h2c]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
+<StructureSection load='5h2c' size='340' side='right'caption='[[5h2c]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5h2c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H2C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5h2c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H2C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H2C FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h2d|5h2d]], [[5h28|5h28]], [[5h2a|5h2a]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h2c OCA], [http://pdbe.org/5h2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h2c RCSB], [http://www.ebi.ac.uk/pdbsum/5h2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h2c ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SWH1, OSH1, YAR042W, YAR044W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), NVJ1, VAB36, YHR195W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h2c OCA], [http://pdbe.org/5h2c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h2c RCSB], [http://www.ebi.ac.uk/pdbsum/5h2c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h2c ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5h2c" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Oxysterol-binding protein homolog|Oxysterol-binding protein homolog]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Baker's yeast]]
+[[Category: Large Structures]]
 [[Category: Im, Y J]]
 [[Category: Manik, M K]]

5h2c

From Proteopedia

Revision as of 06:50, 20 May 2020

Crystal structure of Saccharomyces cerevisiae Osh1 ANK - Nvj1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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