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5h4y

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Revision as of 06:52, 20 May 2020

Crystal structure of human synaptotagmin 5 C2A domain

Structural highlights

5h4y is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	5h4z
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SYT5_HUMAN] May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface (By similarity).

Publication Abstract from PubMed

Synaptotagmins constitute a family of multifunctional integral membrane proteins found predominantly on vesicles in neural and endocrine tissues. 17 isoforms of synaptotagmin family in mammals have been identified, 7 isoforms among them are known to be able to bind Ca2+ via their C2 domains. This study presents the crystal structure of the first C2 domain (C2A domain) of synaptotagmin 5 complexed with Ca2+ at 1.90A resolution. Comparison of the Ca2+-binding pocket of synaptotagmin 5 C2A domain with other synaptotagmin C2 domains demonstrated that a serine residue locating at Ca2+-binding loop probably responsible to the conformational variation of Ca2+-binding pocket, and thus impacts the Ca2+-binding mechanism of C2 domain, which is verified by structural analysis of the serine mutant and Ca2+-binding assays via isothermal titration calorimetry. Alteration of Ca2+-binding mechanism might be correlated with different Ca2+ response rates of synaptotagmins, which is the basis of the functions of synaptotagmins in regulating various types of Ca2+-triggered vesicle-membrane fusion processes.

Structural analysis of Ca2+-binding pocket of synaptotagmin 5 C2A domain.,Qiu X, Ge J, Gao Y, Teng M, Niu L Int J Biol Macromol. 2016 Oct 25. pii: S0141-8130(16)32153-5. doi:, 10.1016/j.ijbiomac.2016.10.083. PMID:27793683^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Qiu X, Ge J, Gao Y, Teng M, Niu L. Structural analysis of Ca2+-binding pocket of synaptotagmin 5 C2A domain. Int J Biol Macromol. 2016 Oct 25. pii: S0141-8130(16)32153-5. doi:, 10.1016/j.ijbiomac.2016.10.083. PMID:27793683 doi:http://dx.doi.org/10.1016/j.ijbiomac.2016.10.083

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5h4y"

@@ Line 1: / Line 1: @@
 ==Crystal structure of human synaptotagmin 5 C2A domain==
-<StructureSection load='5h4y' size='340' side='right' caption='[[5h4y]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='5h4y' size='340' side='right'caption='[[5h4y]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5h4y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H4Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H4Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5h4y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H4Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H4Y FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h4z|5h4z]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h4y OCA], [http://pdbe.org/5h4y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h4y RCSB], [http://www.ebi.ac.uk/pdbsum/5h4y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h4y ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h4y OCA], [http://pdbe.org/5h4y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h4y RCSB], [http://www.ebi.ac.uk/pdbsum/5h4y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h4y ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 5h4y" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Gao, Y]]
 [[Category: Niu, L]]

5h4y

From Proteopedia

Revision as of 06:52, 20 May 2020

Crystal structure of human synaptotagmin 5 C2A domain

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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